Study On Preparation And Property Of Bioactive Treatment And HAP Biomaterial Coating On Titanium Alloy

Titanium (Ti) alloy is widely used as dental implant material due to its good biocompatibility. However, it should be noted that there is a serious corrosion occurred after long-term application. Therefore, Ti alloy should be modified before use for improving its implanting behavior.In this thesis, the corrosion performance of Ti alloy in artificial saliva (AS), saline solution (NS), simulating body fluid (SBF) was firstly investigated by electrochemical method. Ti alloy was bioactive after pretreatment by microarc oxide (MAO) or the improved alkali-heat (IAH) treatment. Then, the samples were immersed into the prepared SBF for preparing HAP biomaterial coating. The analyses of morphology, composition and elements were performed by scanning electron microscopy (SEM), X-ray diffraction (XRD) and energy dispersive spectrometer (EDS). The attachment rate, proliferation and ALP of 3T3-E1 osteoblast cells on sample surfaces were tested. The biocompatibility of the prepared HAP biomaterial coating was also evaluated by animal acute and subacute toxicity test, oral mucosa test, micronucleus test and blood compatibility test, respectively. The main results are drawn as follows:the corrosion of Ti alloy was occurred in three simulating body fluid, and the most serious corrosion is in AS. The HAP biomaterial coating made by MAO has single component with relatively regular shape and compact structure. The adhesion of HAP biomaterial coatings was higher than that of the untreated Ti alloy. Tests of cell proliferation and ALP show that 3T3-E1 osteoblast cells on HAP biomaterial coating is significantly higher than that of the untreated Ti alloy. Animal experiments indicate that, for HAP biomaterial coating made by MAO or IAH, there is no short-term toxicity, oral mucosa irritation, teratogenicity and carcinogenicity, exhibiting the good blood compatibility. As a result, the prepared HAP biomaterial gradient coating made by MAO or IAH treatment shows good bio-security and biocompatibility, which will find promising potential as a new oral implanting material. This will provide theoretical foundation for rational application of titanium alloys in clinic.