The Comparison Of Adipose-derived Stromal Cells And Bone Marrow Mesenchymal Stem Cells Induced Into Neural Cells

Objective:Both the ADSCs and BMSCs have the potential of differentiating into neural cells. Investigate the draw materials,primary culture and serial subcultivation,the identification of stem cells phenotypes between ADSCs and BMSCs, and compare their difference of isolation cultruration,proliferation and the best induction time,the best induction dose,the induction positive rate in differentiation. To investigate the difference between the ADSCs and BMSCs in vitro cultivation and differentiation into Neural cells. Both ADSCs and BMSCs have the potential of differentiating into neural cells. We will choose the best seed cell, meantime,to apply the guarantee to curing the central nervous system diseases.Methods:This study was carried out in the key lab of biochemistry and molecular biology, Shan xi medical university. ADSCs was extracted from subcutaneous adipose tissue of vista rat provided by physiology department. BMSCs was extracted from the bone marrow of the same rat's limbs, which was isolated, cultured and proliferation in vitro.the expression of CD34,CD44, CD45, CD90 and CD 105 on the surface of stem cells was detected by flow cytometry. The growth of the two cells was evaluated by MTT assay graphed in growth curve. The stem cells was induced into three or five passage by 5 mmol/LBME and 2%DMSO or different concentrations of bFGF(5ng/mL,20ng/mL,40ng/mL). The changes of the cells before and after induced were observed by inverted microscope and immunocytochemistry. The expression of NSE,MAP2 on the surface of cells after differation were observed by immunocytochemical method. Demonstrate the differentiative result of ADSCs and to calculate their differentiative rate.Results:The growth of ADSCs was faster than BMSCs. their cell surface marker was CD44(+),CD90(+), CD105(+),CD34(-),CD45(-),the induced rate of low concentration bFGF was significant lower. The mature neurocyte with high concentration was NSE,MAP2 positive. The optimum induced concentration of BMSCs was 20ng/ml, while the ADSCs was 40ng/ml. bFGF was superior to the general chemical inducers. The positive induced rate of ADSCs excelled BMSCs.Conclusion: 1. Both ADSCs and BMSCs have multipotentiality. and their surface marker were similar. They can be induced differentiation into neurocyte.2. The source of ADSCs was more extensive than BMSCs, the ADSCs have shorter incubation time,more optimum multiplication capacity. They can be cultured easily and they are not to be aged.3. In the special induced conditions, the positive induced ratio of ADSCs was higher than BMSCs. The adipose tissue was a new source of Mesenchymal Stem Cells...