Comparison Of Mouse Adult Stem Cells Induced Into Hepatocytes In Vitro And Experimental Study On The Compatibility Between Silk Fibroin Fibers And Adult Stem Cells

Objective1.Incubate and identify the ADSCs and BMSCs, compar the morphology,the ability of prolifertion and differentiation of these two kinds of cells.2.Compare the biocompatibility between these two kinds of cells and Regenerated Silk Fibroin (RSF) material in vivo and vitro and the ability of differentiation into hepatocyte of these two kinds of cells.3.Compare the effect of treatment among the different groups on the carbon tetrachloride acute liver injured mice.Methods1.The ADSCs and BMSCs were incubated and cultured. The morphology, viability, surface makers, and differentiation of two kinds of these cells were detected and confirmed by observation and many associated experiments.2.The colonization and biocompatibility among the two kinds of cells, RSF material and body tissue were detected by HE staining, SEM and confocal laser scanning microscopy.The CM-Dil labeld ADSCs and BMSCs were inoculated to the RSF material, and this two types of cells-combined -RSF material was transplanted to the surface of live of acute liver injured mice.3.Two kinds of cells were injected into acute liver injured mice via tail vein. The RSF material and material with two types cells were transplanted into the acute liver injured mice. The effect of treatment among the different groups were compared by the detection of liver fuction and the result of liver tissue HE staining.Results1.The Real time-PCR, Western Blot and Immunofluorescence staining experiment have showed that both ADSCs and BMSCs could be induced to hepatocyte-like cells.2.The biological characters of ADSCs and BMSCs were not influenced by the RSF material, and these two kinds of cells could survive on this material. The two types of cell-combined-RSF material was rapport with liver and was benefit to the growth of novel vessels and liver cells.3.The colonization of ADSCs and BMSCs on the liver was observed on the 1st,2nd,3rd, 7th days after tail-vein injection by fluorescence microscopy. The effects of treatment were observed on two kinds of cell groups and two types of cell-combined-RSF material group, and the later method was the best one.Conclusion1.The ability of proliferation of ADSCs and BMSCs were resemble, both two kinds of these cells can be induced to hepatocyte-like cells.2.RFS material was rapport with ADSCs and BMSCs, which can be planted on this material long period. The material was also rapport with liver and muscle tissue.3.The dysfunction of liver from acute liver injured mice can be relieved after two kinds of adult stem cell transplantation or two types of cell-combined-RSF material repair, and the later method was the best one.4.Two types of cell-combined-RSF material is superior human made biological hepatic system.