Objective:To detect and analyze the difference of Typeâ… collagen,Typeâ…¢collagen, total amount of collagen(typeâ… +typeâ…¢) and the ratio of collagen typeâ… /â…¢that origin from skin, anterior rectus sheath and transversalis fascia in adult patients with inguinal hernia and without hernia. Methods:we collect 20 patients with inguinal hernia and 20 patients with acute appendicitis operation(control group). Matched-pairs design was used based on ages,sex,tribe and history of smoking.To remove the factors that can induce abnormal metabolism of collagen.All patients' tissue specimens were obtained at the end of operation from skin, anterior rectus sheath and transversalis fascia. All specimens were confirmed by staining with H.E.The contents of collagen typeâ… /â…¢were detected using immunohistochemical methods and the system of computer image analysis. The data were presented as gray level.The lower of gray levels and the more content of collagen in tissue. The total amount of collagen is sum of Typeâ… and Typeâ…¢. Consequence data were expressed by x±s and statistical analysed by SPSS 17.0, size of test was a=0.05. To investigate the difference of Typeâ… collagen,Typeâ…¢collagen, total amount of collagen(typeâ… +typeâ…¢)and the ratio of collagen typeâ… /â…¢between two groups. Results: The inguinal hernia group had less typeâ… collagen, more typeâ…¢collagen, more total amount of collagen and lower ratio of typeâ… /â…¢than control group. Conclusion:Inguinal hernia can be regarded as a disease of systematic collagen disorder and a local manifestation of a systematic collagen disorder.
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