Investigation Of The Effects Of Electrode On Expressions Of ATP5B And Cytc In Penicillin-induced Epileptic Rat

Objective Mechanisms of bioenergy change were investigated during epileptic seizures in penicillin-induced epileptic rat brains and the effects of electrode interfering with it by observing the changes of behavior, EEG, epileptogenic focus potential, and the expressions of ATP5B and Cytc in hippocampus neurons of penicillin-induced epileptic rats.Methods Using brain stereotactic apparatus, the acute seizure model was prepared on the right hippocampus of rats microinjected with penicillin.50 Sprague-Dawley (SD) rats were randomly divided into 4 groups, namely normal saline control group (group A), penicillin model group (group B), metal electrode interfere group (group C) and insulated electrode interfere group (group D). Saline was only microinjected on the right hippocampus in 5 rats for Saline control group. Penicillin was microinjected on the right hippocampus in 15 rats of group B that was divided into the 1st hour,3rd hour and 24th hour three subgroups; in group C and D, metal electrode or insulated electrode was implanted into the foci of microinjection for the goal of interfere after the epileptic seizure model was made. The latency period and amplitude of seizure, the waveform characteristic and epileptic wave frequency on EEG, the epileptogenic focus potential and expressions of ATP5B and Cytc in hippocampus neurons were observed.Results The seizure amplitudes reached the pick at the 3rd hour in penicillin-induced epileptic rats, and the latency period was shorter in group C than that in group B; epileptic discharge wave could be recorded on the EEG in group B, C and D, and the frequency of epileptic waves were decreased after metal electrodes were implanted; the trend of epile-ptogenic focus potential increased slowly to a pick at the 3rd hour and then decreased to baseline, and the amplitudes of epileptogenic foci potential were reduced after metal electrodes were implanted; the expressions of ATP5B and Cytc in hippocampus neurons showed the trend that increased at 1 hr, and decreased at 24 hr, and the metal electrodes implanted had no effect on this trend. Conclusions The expressions of ATP5B and Cytc in hippocampus neuron of penicillin-induced epileptic rat showed the evidence that bioenergy had participated in the process of epileptic seizure; the change of epileptogenic foci potential was found and it could be reduced by implantation of metal electrode possibly through the mechanism of conducting.