| Objective To investigate the effect of allicin on LPS stimulated human periodontal ligament cells'apoptosis and inflammatory cytokines production. Methods Human periodontal ligament cells were cultured and identified in vitro by explant method. (1)MTT was used to detect the suppression effect of allicin and LPS on hPDLCs.The morphological change was observed by AO/EB staining technique. Quantitative analysis of apoptotic cells were evaluated by flow cytometry. (2)the expression of IL-1βTNF-αinduced by allicin and LPS on HPDLC was examined by enzymelinked immunosorbent assay (ELISA). Results (1)The inhibition effect of LPS on hPDLC increased when the concentration of LPS was within 1μg/ml-100μg/ml. Allicin had suppression effects on hPDLCs, and such effect was allicin-concentration-dependent. (2)AO/EB staining revealed that control group showed normal morphology and green coloration of cytoplasm and cell nucleus. The experimental group, that is, incubated with LPS and/or allicin, displayed abnormal morphology and color. (3)Total apoptosis rate and that of early stage in allicin groups were obviously enhanced compared with control group through flow cytometry technology(P< 0.05). LPS groups had higher total apoptosis rate and that of early and late stage(P< 0.05). Compared with LPS groups, LPS+3μg/ml allicin could raise the total apoptosis rate(P< 0.05);LPS+6μg/ml allicin decreased the apoptosis rate of each stage(P> 0.05); LPS+9μg/ml allicin increased the late and total apoptosis rate(P< 0.05). (4)TNF-αand IL-1βsecretion were increased when hPDLCs were incubated with LPS or allicin. However, treating with LPS and allicin could decrease such effect. Conclusions (1) LPS could raise the apoptosis rate of hPDLCs. (2) Allicin of low concentration could suppress the apoptosis induced by LPS, however, high concentration would enhance. (3) Allicin could suppress the production of TNF-αinduced by LPS. (4) Allicin could suppress the production of IL-1βinduced by LPS.
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