The Effect Of Curcumin On Expression Of Survivinin Balloon Injury Of Rat Carotid Arteries

Objective:Proliferation of vascular smooth muscle cells (SMCs) have been implicated in the healing response to vascular injury process. We sought to investigate the role of survivin (SVV), a factor of cell proliferation, in lesion formation after vascular injury. The other objective of this study was to determine the effect of Curcumin on cell proliferation in the model. Methods:Fifty four male SD rats were randomly divided into three groups:sham operation group; vascular injury group; curcumin-treated group. Model of carotid artery injury was established.The sham operation group and vascular injury group was given intragastric administration of normal saline. Curcumin was given in Curcumin-treated group (100 mg/kg/d).In the three groups, carotid arteries of rat was extracted at 3th,7th,14th and 28th.The expressions of survivin and proliferating cell nuclear antigene (PCNA) proteins in target vessels were assessed by immunohistochemisty method. Fluorescence-based real-time reverse tras-cription PCR was used for quantifacion of mRNA of survivin,PCNA. Interleukin-18(IL-18) in serum was assessed by enzyme linked immunosorbent assay.Results:1. survivin protein expressed on 7th,14th in vascular injury group significantly higher than that of 7th,14th in curcumin treated group and sham operation group (p< 0.05)2. survivin mRNA expressed in vascular injury group significantly higher than that of curcumin treated group and sham operation group (p< 0.05).PCNA mRNA expressed in vascular injury group significantly higher than that in sham operation group and 7th,14th curcumin treated group (p< 0.05).The expression of IL-18 in 3th,7th and 14th in vascular injury group was significantly higher than that of curcumin treated group and sham operation group (P<0.05)3. The expression of IL-18 in 3th,7thand 14th vascular injury group was significantly higher than that of curcumin treated group and the sham operation group (P<0.05)Conclusion:Survivin promoted proliferation of vascular endothelial cells and smooth muscle cells. Curcumin down-regulates expression of survivin to inhabite the proliferation of vascular smooth muscle cells. IL-18 promoted proliferation of smooth muscle cells. Curcumin inhabited expression of IL-18 to inhabite the proliferation of vascular smooth muscle cells.