| Objective To investigate the relationship between cell apoptosis and expression of thrombin in the perihematoma tissue in early hypertensive intracerebral hemorrhage (HICH).Methods 36 samples were obtained from the perihematoma region of HICH patients and divided into 5 groups according to the time from onset to operation:6hours (n=7),6～12hours (n=11),12～24hours (n=8),24～48hours (n=6),48～72hours (n=4). The normal control group were collected from the patients with lateral ventricle tumor who were operation by transcortical approach.The terminal deoxynucleotidyl-transferase mediated dUTP-biotin nick end labeling (TUNEL) method was used to detect apoptosis and the expression of thrombin was observed by immunohistochemistry.Results the TUNEL positive cells was almost no expression in the control group (0.32±0.037) and it have a slight expression in<6 h group(7.19±2.53,P< 0.05).It increased gradually 6hours after the onset of HICH (15.11±3.69, P< 0.01)and reached the maximum at 24～48 hours (53.79±6.35, P< 0.01).48-72h group has dropped slightly, but still maintain a high level (38.23±3.29, P<0.01). The expression of the thrombin was consistent with the apoptosis. The correlation analysis showed that the TUNEL positive cells was positively correlated to the expression of the thrombin (P<0.05 or P<0.01).Conclusion Apoptosis cells exist in perihematomal region among hypertensive intracerebral hemorrhage.The expression of thrombin is a critical step in apoptotic cell death after hypertensive intracerebral hemorrhage.
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