HIF-1α And VEGF In Rabbit Endothelial Cells Of Vein Graft Re-endothelialization Of Expression

Hypoxia-inducible factor-la (HIF-la), vascular endothelial growth factor (VEGF) expression in angiogenesis of significance, HIF-la is a hypoxia state the core of regulation of angiogenesis factors, by influencing other growth factors Expression of the entire process involved in angiogenesis. VEGF is a critical early stage of angiogenesis growth factor, hypoxia may induce substantial expression of VEGF.VEGF is a critical early stage of angiogenesis growth factor, hypoxia may induce substantial expression of VEGF. VEGF in the angiogenesis play an important role, VEGF has vascular endothelial cell division, proliferation, increase vascular permeability, induce endothelial cell Bcl-2 gene expression, enhance the ability of endothelial cells in anti-apoptotic effect.In this study, by detecting hypoxia-inducible factor-la (HIF-la) and vascular endothelial growth factor (VEGF) in rabbit carotid artery endarterectomy +anterior vein autogenous vein graft and postoperative phases of expression and the vein graft after exfoliative endometrial changes in segmental artery lumen cross-sectional area to study the HIF-1αand VEGF in a rabbit vein graft re-endothelialization role in the process.ObjectiveTo investigate the HIF-1αand VEGF expression in pairs of rabbits vein graft re-endothelialization of impact.1,HIF-la and VEGF in the rabbit carotid artery endarterectomy+anterior vein autogenous vein graft and postoperative phases of the expression of the relationship.2,after stripping of vein graft intimal changes in segmental artery lumen cross-sectional areaMethods1,group and the production of 6-month-old animal model 46 adult male New Zealand white rabbits were randomly divided into two groups, the first group of 10, the second group of 36 (including 30 for the trial to be animals, six of standby). Are based on ordinary food a week after feeding, collecting the first group of 10 rabbits were used in serum by enzyme-linked immunosorbent assay (ELISA) line of HIF-la and VEGF detection, and then take all 10 rabbits left cervical venous line HE staining do pathological examination to observe the arterial lumen area. The second group of 36 rabbits underwent left common carotid artery endarterectomy+ anterior vein autogenous vein graft, producing restenosis model,An ordinary diet after feeding.7 days after the operation,14 days,30 days followed by three time points were randomly selected from 10 rabbits collected serum by enzyme-linked immunosorbent assay (ELISA) line of HIF-la and VEGF testing, blood tests after taking the 10 rabbits in paragraph artery intima stripping HE staining did pathology laboratory, and to observe the arterial lumen area, the number of intimal hyperplasia and smooth muscle dynamics.2,pathological image analysis image analysis system using pathological specimens:Rabbit vein graft HE staining, Mason morphological analysis of stained films.3,chemical analysis of vein graft, respectively, prior to surgery and 7 days after 14 days,30 days, blood serum by ELISA measurement of HIF-1αand VEGF levels.Results1,HIF-la and VEGF expression in each period with a significant correlation (P <0.01); HIF-1αand VEGF in the rabbit autologous vein graft expression was significantly increased (P <0.01), in which the highest in 7 days, followed by a gradual decreased 30 days after the operation is only slightly higher than normal.2,HE staining showed intimal denudation arterial segment lumen area (LA) was the first overall diminishing, at the lowest value reached after 14 days and then gradually increasing trend.Conclusion1,this experiment has successfully produced a rabbit carotid artery endarterectomy and autogenous vein graft restenosis model.2,tissues and organs in acute hypoxia-ischemia period of HIF-la and VEGF expression increased rapidly; with prolonged ischemia and hypoxia of tissues and organs adapt gradually reduced two-factor concentrations, this experiment the observed HIF-la and VEGF expression peaked at 7 days after operation.3,after vein graft vascular remodeling in the early HIF-1αand VEGF can promote damage to the high expression of vascular endothelial cells (VEC) reconstruction of the structure and function to maintain the arterial lumen area; as time goes by, HIF-la and the the expression of VEGF gradually decreased after stripping of vein graft intimal segment arteries gradually narrowed to a minimum of 14 days after the operation, and then gradually increased.4,HIF-la and VEGF expression after stripping peaks and vein graft intimal minimum segment arteries, respectively after 7 days in 14 days, so after 7-14 days of HIF-la and VEGF expression in early reduction may be related to the vein graft restenosis.