Study On Culture Of Circulating Endothelial Cells In Vitro And Endothelialization Of Prostheses EPTFE

Objective: To discuss the endothelialization of artificial vessel with convenient endothelial cells. Methods: Umbilical cord blood monocytes were isolated by centrifugation, cells were propagated in EC growth media, and developing cultures were observed by optical and scanning electron microscopy . Endothelial cells were immunostained for qualitative assay and flow cytometry studied for quantitative assay. ECs were lined to ePTFE prosthesis by rotation adherent culture.Results: The progenitors were proliferated by up to 3.6 + 1. 7, which transduced to endothelial cells at 5 days during culture, and kept proliferation within 2 weeks. Immunostaining of EC was positive for CD3U CD34s factor VIII and VEGFR-2 at premature in culture, which was negative for all but factor VIII at late culture. Morphological observation: EC had the typical cobblestone appearance, and monolayer culture , EC were lined to ePTFE prostheses by rotationadherent culture.Conclusion: We conclude that the circulating endothelial cells can be induced and propagated during culture in vitro, and also used for endothelialization of artificial vessel in vivo.