The Study On The Preparation And Pharmacodynamic Action Of The Delayed Release Gel Of Stabilized Chlorine Dioxide

Objective:In the paper,the stabilized chlorine dioxide as a model drug,water-based gel matrix as the main substance,prepared the topical external preparation-the delayed-release gel of stabilized chlorine dioxide which extended the role of the effect,reducing the number of drug use on the prevention and treatment of various types of traumatic infection.Method:The UV spectrophotometry and ion-pair reversed-phase high performance liquid chromatography were established to determine the assay of the stabilized chlorine dioxide and chlorine dioxide gel. The dermination of chlorine dioxide gel for sustained release in vitrode was determined by UV spectrophotometry. In the blank gel matrix screening process,the acid-soluble chitosan, carboxymethyl cellulose sodium and Carbopol 940 were selected with a systematic screening.in this foundation, the release rate as a target, filtered out the best prescription gel. The quality evaluation, release properties in vitro and stability were carried out, the antimicrobial study was also carried out with the E. coli, Staphylococcus aureus, Bacillus subtilis as a representative of the bacteriostatic. The effect of the prevention and treatment of wound infection study was carried out with the rabbit skin wound as a model.Result:At the wavelength of 260nm, the chlorine dioxide in the gel was determined by UV spectrophotometry, the average recovery rate was 99.20%, RSD was 1.07%, the determination was accurate and reliable. At the same time,the article also explored to use ion-pair reversed-phase high performance liquid chromatography of which mobile phase included the Tetrabutylammonium hydroxide ion-pair reagent to determin the chlorine dioxide in the sustained-release gel. The average recovery was 99.36%, RSD was 1.24%. Results are accurate. The results of the method and UV spectrophotometry are consistent. In the selection process,the stability of the acid-soluble chitosan chlorine dioxide release gel remains to be further studied and improved. With sodium carboxymethyl cellulose and Carbopol 940 as the main mixing matrix of slow-Release Gel were in line with the requirements. The prescription contained mainly:the stabilized chlorine dioxide, sodium carboxymethyl cellulose, carbomer 940, acetic acid, glycerol and so on. In the pharmacodynamic action,the minimum inhibitory concentration for Escherichia coli and Bacillus subtilis was 74.07mg/L, for Staphylococcus aureus was 9.26mg/L. The minimum bactericidal concentration for Escherichia coli and Staphylococcus aureus was 148.14mg/L.The therapeutic effect of chlorine dioxide gel on the rabbit back injury was consistent with mupirocinConclusion:The method of UV spectrophotometry for determining the chlorine dioxide was simple and low-cost. The ion-pair reversed-phase high performance liquid chromatography for determining chlorine dioxide was accurate, simple and high sensitivity. The quality of the slow-release of chlorine dioxide prepared with sodium carboxymethyl Cellulose and carbopol 940 as the main mixed-matrix consistent with the 2005 edition of Chinese Pharmacopoeia. One, chlorine dioxide gel composition A, second, activator gel component B, clinical use pre-mixed evenly. Slow-release gel of chlorine dioxide was broad-spectrum antibacterial activity. The result of the back injury of treatment in rabbits as a model was satisfactory. As a topical application prescription could achieve the prevention and treatment of various types of traumatic facial infection.