| Ketamine hydrochlorid (KH) is a common ftupetacient in clinical medicine and it is also one kind of new drug. On the one hand it relieves pain in surgical procedures, on the other hand it brings serious damages to social and drug addicts. So it has very important realistic significance to detect patients who were injected ketamine hydrochlorid and the ketamine hydrochlorid contents in drug addicts. At present, there are many methods to detect it such as: chemical staining, chromatography, spectroscopy, immunoanalysis, potentiometry. The ionic selective electrodes (ISE) have a great development due to its simple, rapid and suitable for field analysis. However, the selectivity and sensitivity of the ISE are not good enough. After reviewed the works in references, the following research works were done in this dissertation.1. Study on the Mechanism of Potential Response of a Ketamine Sensitive Membrane ElectrodeIran scholar alizadeh has published an article in Journal of Pharmaceutical and Biomedical Analysis in 2002. In the article, they reported a membranous ketamine ionic selective electrode (ISE) with PVC as matrix to determinate ketamine hydrochlorid, however, the results in the original literature show that the ISE selectivity was very poor. This chapter points out that the original literature misapprehensively understood the mechanism of potential response, explains the causation of why its selectivity was very poor, and reports the response mechanism of the sensitive membrane. Our experimental results show that the original literature based on ketamine hydrochlorid ion-exchange response mechanism, which is not quite incomplete. The response of the PVC-KH membrane electrodes toward KH includes infiltration induced by plasticizer and charging situation of membrane and ion-exchange of carboxylated PVC (PVC-COOH) carboxylated PVC-KH complexes (PVC-COO-KH). But the plasticizer induced infiltration acted as the primary factor to generate a higher but poor selective response. 2. Improvement of a CHI-400A electrochemical quartz crystal microbalance measurement deviceWe study on ketamine hydrochlorid quartz crystal sensors as they are not reported in references and quartz crystal sensors having high sensitivity and superior selectivity. In our laboratory, as the CHI-400A electrochemical quartz crystal microbalance is instability and can not apply in solution real-time detection. So, this chapter is to modify the device to eliminate the interference caused by electromagnetic noise, mechanical vibration and changes of wafer stress. And the device is connected with sequential injection to constitute a flow micro-testing cell. The experimental results show that the improved device has strong stability and lower noise, the stability of QCM frequency response in the liquid phase is±0.1Hz/min, noise is 0.03 Hz, basically play the best performance of the instrument can be used for solution real-time detection.3. Study on a ketamine hydrochlorid sensitive quartz crystal sensorBased on the first part of our work, the optimized sensitive membrane is coated on the surface of quartz crystal to develop the ketamine hydrochlorid sensitive quartz crystal sensor. The experimental results show that by employing PVC embedding method, the prepared sensitive quartz crystal sensor is difficult to achieve high sensitivity because a lot of polymer coat on the surface of quartz crystal to increase the load. A sensitive ketamine hydrochlorid quartz crystal sensor based on the electropolymerization molecular imprinting polymer with o-aminophenol as monomer and ketamine hydrochlorid as template, and the relevant research work is still in progress.
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