| Polycyclic aromatic hydrocarbons (PAHs) have been received considerable attention for their carcinogenic, teratogenic and mutagenic actions. As the PAHs with highest evaporability, the external naphthalene exposure of the general population in the environment is reported to be high in comparison with other PAHs. Now, naphthalene is reclassified as possible human carcinogen by several national and international agencies. Recently, more studies can confirm that using 1-naphthol (1-NAP) and 2-naphthol (2-NAP) in urine as biomarker for PAHs is possible. Up to now, the existing methods for the determination of 1-NAP and 2-NAP have disadvantages of high cost, long analysis time, complex sample preparation and derivatization pre-procedures, and. they can not be used for monitoring in-suit. So, there is important practical significance to develop novel methods for determination of the metabolites of 1-NAP and / or 2-NAP.In chapter 2, a new method was developed by quartz crystal microbalance (QCM) technique coupled with K-matrix in stoicheiometry for simultaneous determination of trace 1-NAP and 2-NAP in urine samples. Our investigation shows that in the B-R buffer solution (pH=6.37), 1-NAP and 2-NAP formed a 1:1 stoichiometric ratio complex compound withβ-CD, which was adsorbed on nanometer TiO2 films of the QCM surface, resulting in descending of the frequency values (ΔF). The 1-NAP and 2-NAP embedded in the hydrophobicity cavity ofβ-CD can emerge some differents because their enter way were different, by which the isomers can be distinguished by K-matrix method in stoichiometric.The frequency shift values of QCM (ΔF) were in proportion of the concentration in the range of 2.75×10-6~1.30×10-4 mol L-1 and 7.16×10-7~1.20×10-4 for 1-NAP and 2-NAP, respectively. The detection limits were 8.36×10-7 mol L-1 and 2.15×10-7 mol L-1 for1-NAP and 2-NAP, respectively. The average recovery was 113.0 %~133.0 %. This method was simple, quickly, high sensitive and accurate. It had been applied to the determination of trace naphthols in human urine, and the obtained results were in good agreement with those obtained by the method of high performance liquid chromatography.In chapter 3, the interaction between TiO2 andβ-CD and the clathration ofβ-CD and naphthol were studied by ultraviolet absorption spectrum, IR spectra,XPS and TG analysis spectra. The clathration ofβ-CD formed clathrate with naphthol in different pH values was determinationed by fluorometric method, the association constant K ofβ-CD complex with 1-NAP and 2-NAP had determination also. Results above were discussed.In chapter 4, a new method was developed for the determination of 2-NAP by using wireless magnetoelastic sensing technologies. In the dilute hydrochloric acid medium, sulfamethoxazole reacts with cevit to produce a diazo compound in the icewater bath at 0°C. The diazo reaction product reacts with 2-NAP at alkalinity condition and the salmon pink sediment was obtained, resulting in descending of the frequency values. The frequency shift values (ΔF) of wireless magnetoelastic sensor was in proportion of the concentration in the range of 1.13~138.88μmol L-1 for 2-NAP with the coefficient correlation of 0.9970 and the detection limits of 0.34μmol L-1. The relative standard deviation was 2.4 % and average recovery was 107 % (n=6).
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