Expression Changes Of Toll-like Receptor 5 MRNA In Peripheral Blood Mononuclear Cells In Coronary Heart Disease Patients

Objective: Many study suggested that inflammation plays a crucial role in initiation, evolution and rupture of atherosclerotic plaque of coronary heart disease(CHD)patients. The Toll-like receptor(sTLRs),a main receptor of native immune system identifying pathogenic microorganisms, plays an important role in the native immune reaction. It is the bridge of innate immune and adaptive immune response. The purpose of this study is to investigate the expression of TLR5,NF-κB and in peripheral blood mononuclear cells(PBMCs)in CHD patients and to evaluate the value of TLR5 signaling pathway in CHD patients.Methods: The 54 patients was diagnosticated coronary artery disease(CAD).The clinical diagnosis was according to typical clinical manifestation, myocardium enzymology, electrocardiogram and so on. Corresponding with the coronary artery disease diagnostic criteria of World Health Organization(WHO).10ml Heparinized blood was obtained from 54 CHD patients (within 24 hours),meanwhile 15 health volunteers serve as control. Total RNA was isolated from these patients'mononuclear cells and volunteers'.RT-PCR was used to detect TLR5 usingβ-actin as internal index. The expression of TLR5 in PBMCs was measured by PT-PCR in 17 patients with acute myocardial infarction(AMI), 19 patients with unstable angina pectoris(UAP), 18 patients with stable angina pectoris(SAP), and 15 controls. The level of serum TNF-αwas detected in the above-mentioned 4 groups by Enzyme-linked immunsorbent assay(ELISA).Result: The expressions Mrna of TLR5 inPeripheral Blood Mononuclear Cells were increased in CHD patients than that of in health volunteers. The TNF-α, which was significantly positive correlated with TLR5 level,was higher in CHD patients than that of in health volunteers.Conclusion: The expression of TLR5 in PBMCs is higher in CHD patients. The inflammation reaction mediated by TLR5 may participate in the pathogenesis of CHD.