Effect Of Ethanol On Human Umbilical Vein Endothelial Cells And Protective Effect Of Fenofibrate

Objective:To investigate the injury of human umbilical vein endothelial cell by ethanol in vitro. To observe the protective effect of Fenofibrate on human umbilical vein endothelial cell under the induction of ethanol.Methods:1. The injury of human umbilical vein endothelial cell induced by different concentrations of ethanol in vitro: HUVEC were divided into normal control group and ethanol injury group with different concentration(0.05mmol/L, 0.1mmol/L, 0.5mmol/L,1mmol/L, 5mmol/L, 10mmol/L, 25mmol/L,50mmol/L, 100mmol/L, 200mmol/L,500mmol/L,1000mmol/L),they were incubated 24h, the levels of MTT in each group were measured, and maleic dialdehyde(MDA),superoxide dismutase(SOD),nitric oxide(NO),intercellular adhesion molecule-1(ICAM-1)and apoptosis rate were also measured in normal control group and ethanol group(50mmol/L, 100mmol/L, 200mmol/L).2.The protective effect of fenofibrate on human umbilical vein endothelial cell injury induced by ethanol:HUVEC were divided into normal control group, fenofibarte group(fenofibarte10umol/L+ethanol100mmol/L,fenofibarte50umol/L+ethanol100mmol/L,fenofibarte100umol/L+ ethanol100mmol/L) and VitC group (VitC 100mg/L+ ethanol 100mmol/L).HUVEC were injured by 100mmol/L ethanol after they were incubated with different concentrations of fenofibarte and 100mg/L VitC for 4h.Maleic dialdehyde(MDA),superoxide dismutas(eSOD),nitric oxide(NO),intercellular adhesion molecule-1(ICAM-1)and apoptosis rate were measured.Result:1. HUVEC vitality decreased in ethanol groups, the levels of MDA, ICAM-1 and the apoptosis rate in ethanol groups were significantly higher than normal control group, and the levels of SOD, NO in ethanol groups were significantly lower than normal control group. Ethanol (50mmol/L, 100mmol/L, and 200mmol/L) can inhibit HUVEC vitality; promote the synthesis of MDA, ICAM-1; inhibit the expression of SOD, NO and promote the apoptosis of HUVEC.2. The levels of MDA, ICAM-1 and the apoptosis rate in fenofibrate groups were significantly lower than 100mmol/L ethanol group, and the levels of SOD, NO in fenofibrate groups were higher than 100mmol/L ethanol group. Fenofibrate (10umol/L, 50umol/L, and 100umol/L) can protect HUVEC against injury induced by ethanol as a result of inhibiting the expression of SOD and NO, increasing the production of MDA, ICAM-1 and inhibiting the apoptosis of HUVEC. And these effects are dose-dependent.Conclusion:1. HUVEC injury can be induced by ethanol in vitro.2 .The mechanisms of HUVEC injury induced by ethanol may be closely associated with oxidative stress, inflammatory reaction and apoptosis.3.Fenofibrate can protect HUVECs against injury induced by ethanol, and these effects are dose-dependent.4. The mechanisms of the protection of fenofibrate include the inhibition of oxidative stress, inflammatory reaction and apoptosis.