Study On Expression And Correlation Of SPARC And MMP-2 In Meningiomas

ObjectiveExamining the expression of SPARC and MMP-2 in meningiomas, investigating the relation of SPARC and MMP-2 with the tumorigenesis, malignant, transformation and invasivenesis in meningiomas, providing theoretical basis for diagnosis, treatment and prognosis of meningiomas.Methods1. Subjects and experimental groups:we studied sixty-two specimens diagnosed as meningiomas of various histological types and of different grades extracted from patients who underwent surgery firstly in the department of neurosurgery of the second Xiang-ya hospital by random during the period from July,2006 to August,2009; none of the patients were treated before operation and had serious disease. Twenty normal brain meninges specimens used as the control group were obtained from patients with acute craniocerebral injury who underwent subtemporal decompression during the period from January,2009 to August,2009 by random, all patients didn't have the history of tumor. The histological slides of all patients were divided into (1) meningioma group:according to the WHO classification of brain tumors in 2000, it were categorized into gradeⅠgroup forty-five cases, gradeⅡgroup eleven cases and gradeⅢgroup six cases; (2) normal brain meninges control group twenty cases.2. SPARC and MMP-2 determination:examining the expression of SPARC and MMP-2 in meningiomas by Immunohistochemistry S-P (streptavidin-peroseidase) method. Immunostaining for SPARC and MMP-2 was evaluated according to staining frequency of tumor cells by multi-function True Color pathological analysis system. The cytoplasm and (or) membrane of the positive cell was stained by brown granules. Five representative fields were selected to evaluate all cases (×400) by random.Results1. The SPARC expression of five cases was positive in normal control group. Positive immunoreactivity of SPARC was expressed in twenty-six cases of the WHO gradeⅠmeningiomas, in ten cases of the WHO gradeⅡmeningiomas and in six cases of the WHO gradeⅢmeningiomas. The SPARC expression of meningioma group was higher than in normal control group, the difference has statistics significance (X2=11.368, P=0.001). This showed that immunoreactivity of the SPARC was significantly higher as histological malignancy gets higher, the difference has statistics significance(X3=7.603, P=0.022).2. Forty-five cases of WHO gradeⅠmeningiomas, fourteen were invasive meningiomas, thirty-one were non-invasive meningiomas. Positive immunoreactivity of SPARC was expressed in thirteen cases of the invasive meningiomas and in thirteen cases of the non-vasive meningiomas; the SPARC expression of invasive meningiomas was significantly higher than the expression of non-invasive group, the difference has statistics significance (X2=19.067, P=0.001).3. The MMP-2 expression of four cases was positive in normal control group. Positive immunoreactivity of MMP-2 was expressed in twenty-one cases of the WHO gradeⅠmeningiomas, in nine cases of the WHO gradeⅡmeningiomas and in six cases of the WHO gradeⅢmeningiomas. The MMP-2 expression of meningioma group was higher than in normal control group, the difference has statistics significance(X2=11.160, P=0.001). This showed that immunoreactivity of the MMP-2 was significantly higher as histological malignancy gets higher, the difference has statistics significance (X2=6.991, P=0.030).4. Forty-five cases of WHO gradeⅠmeningiomas:fourteen were invasive meningiomas, thirty were non-invasive. Positive immunoreacti-vity of MMP-2 was expressed in twelve cases of the invasive meningiomas and in ten cases of the non-vasive meningiomas. The MMP-2 expression of invasive meningiomas was significantly higher than the expression of non-invasive group, the difference has statistics significanc (X2=14.841, P=0.000).5. In sixty-two cases of meningiomas, the expression of SPARC and MMP-2 was significantly positive correlated (r=0.613,P=0.000); in forteen cases of invasive (WHO gradeⅠ) meningiomas, the expression of SPARC and MMP-2 was significantly positive correlated (r=0.696, P=0.000). Conclusion1. The expression of SPARC and MMP-2 in meningiomas was higher than in normal brain meninges; with the rise of pathological grade of meningiomas, the expression of SPARC and MMP-2 increased; in WHO gradeⅠmeningiomas, expression of them in invasive meningiomas was significantly higher than that in non-invasive meningiomas; they possibly played an important role in the tumorigenesis,malignant transformation and invasivenesis of meningiomas.2. The expression of SPARC and MMP-2 was positively correlated in meningiomas and might had close relation in the tumorigenesis, malignant transformation and invasivenesis of meningiomas.3. the expression of SPARC and MMP-2 was positively related In invasive(WHO gradeⅠ)meningiomas, there may be a mutual Synergy, which could enhance the meningioma's invasive ability. the joint detection of the two indicators could be used as objective indicators of invasive meningiomas.