Font Size: a A A

In Vitro Effect Of Resveratrol On Hepatocarcinoma Cell Line Bel-7402

Posted on:2011-10-20Degree:MasterType:Thesis
Country:ChinaCandidate:L P LiFull Text:PDF
GTID:2154360305994597Subject:Biochemistry and Molecular Biology
Abstract/Summary:PDF Full Text Request
Objectives To investigate the effect of resveratrol on the proliferation, apoptosis, and expression level of silent information regulator 1 (SIRT1) of human hepatocarcinoma cell line Bel-7402.Methods Human hepatocarcinoma cell line Bel-7402 was cultured and treated with different concentrations(0,12.5,25,50,100, and 200μM) of resveratrol for 24,48 and 72 hours. The growth inhibition rate of Bel-7402 cells was detected by MTT method. The morphological changes of Bel-7402 cells were observed by the inverted microscope and the fluorescent microscope. The flow cytometry was applied to analyze the cell apoptosis rate and cell distribution in the cell cycle. The expression level of SIRT1, mRNA and protein production, was tested with RT-PCR and Western blotting, respectively.Results Resveratrol inhibited the proliferation of Bel-7402 cells in a time- and concentration- dependent manner ranging from 12.5 to 200μM and from 24 to 72 hours. After 72 hours of treatment, IC5o for resveratrol was 62.84μM; After treatment with 200μM resveratrol, the inhibition rate for Bel-7402 cells was 77.62±1.51%(P<0.05). Both the inverted microscope and the fluorescent microscope showed typical morphological changes in Bel-7402 cells treated with resveratrol. Results of the flow cytometry showed that resveratrol induced Bel-7402 cell cycle is mainly with S-phase arrest. After treatment with the concentration of 0,12.5,25,50,100, and 200μM resveratrol for 24 hours, the apoptosis rate of Bel-7402 cells was 2.56%,16.8%,20.4%,35.8%, 38.0%and 42.3%, respectively, the expression level of SIRT1 mRNA and SIRT1 protein production was significantly increased (P<0.01), and reached the peaked at the concentration of 25μM resveratrol treatment, then declined gradually as resveratrol concentration level increases.Conclusions Resveratrol can inhibit the proliferation and induce the apoptosis of Bel-7402 cells in a time- and concentration-dependent manner by increasing the expression level of SIRT1.
Keywords/Search Tags:resveratrol(Res), hepatocarcinoma, silent information regulator 1 (SIRT1)
PDF Full Text Request
Related items