Study On Relationship Between Genetic Polymorphisms Of CYP1A1 And GSTM1 Gene And The Susceptibility To Teratospermia With Smoke

PURPOUS Analysis the pertinence between the genomic polymorphism of two metabolic enzymes "CYP1A1" & "GSTM1" and the Genetic Predisposition of Teratospermia which caused by smokingMETHOD A group of 60 teratospermia specimens of sterile patients who were already approved had Teratospermia was collected in "the Second Xiangya Hospital of Central South University" from 2010/3/1 to 2010/5/1; Meanwhile, another so called "comparison group" of 60 specimens of sterile patients whose sperms were approved as natural was collected too. Use PCR-RFLP to examine the distribution frequency of genomic polymorphism of CYP1A1 & GSTM1 of both "teratospermia group" and "comparison group", analyze the pertinence of the two metabolic enzymes and teratospermia, and, the interaction between two metabolic enzymes ("CYP1A1" & "GSTM1") and the Genetic Predisposition of Teratospermia which caused by smoking.RESULTS1. Distribution frequency of CYP1A1 "teratospermia group" and "comparison group" is wild type 43.3%, hybrid type 23.3%, mutant 33.3%and 43.3%, genotype 43.3% and 11.7%, the comparison of frequency differences of two groups has statistical difference (X2=9.88,P=0.007); Distribution frequency of GSTM1 "teratospermia group" and "comparison group" GSTM1 (+)和GSTM1 (-) is 30%,70% & 53.3%,46.7%, the comparison of frequency differences of two groups has significant statistical differences (X2=6.72,P=0.01)2. Take CYP1A1 wild type as reference, CYP1A1 heterozygous genotype OR=0.48 (95%CI=0.20-1.15), no significant statistical differences (P=0.95); CYP1A1 mutant type gene OR=2.97 (95%CI=1.08-8.19), has significant statistical differences (P=0.03); Combined CYP1A1 heterozygous genotype and CYP1A1 mutant gene, the dangerous degree has increased, but it has no significant statistical differences (OR=1.07,95%CI=0.52-2.20, P=0.85)3. Take GSTM1 (+) as reference, GSTM1 (-) OR=2.667, 95%CI=1.26-5.64, has significant statistical differences (P=0.01)4. Take CYP1A1 wild type from non-smoking person as reference, a non-smoking person who carries CYP1A1 heterozygous genotype doesn't have more danger to get teratospermia, a non-smoking person who carries CYP1A1 mutant type gene has more danger to get teratospermia, but it has no significant statistical differences.The dangerous degree in a smoking person who carries CYP1A1 wild type gene was 3.5 times as much as that (P=0.04); The dangerous degree in a smoking person who carries CYP1A1 heterozygous genotype was 2.4 times as much as that (P=0.19); CYP1A1 mutant gene was 5.3 times (P=0.02); Combined CYP 1A1 eterozygous genotype and CYP1A1 mutant gene was 3.2 times (P=0.54)。5. Take GSTM1(+),CYP1A1 wild type as reference. The dangerous degree in a person who carries GSTM1(+) and CYP1A1 mutant type gene was 1.24 times as much as that (95%CI=0.23-6.62), no significant statistical differences (P=0.8), But the dangerous degree in a person who carries GSTM1(-) and CYP1A1 mutant type gene was 3.71 times as much as that (95%CI=0.98-14.05), has significant statistical differences (P=0.03)。CONCLUSION1. Take a normal person to get teratospermia as reference, The dangerous degree in a person who carries CYP1A1 mutant gene has increased.2. Take a normal person to get teratospermia as reference, The dangerous degree in a person who carries GSTM1 (-) has increased significantly.3. Take a normal person to get teratospermia as reference, The dangerous degree in a person who carries both GSTM1 (-) and CYP1A1 mutant gene has increased significantly.