Preliminary Study Of Tissue Engineering Skins Construction By Scalp Cells Combined With Artificial Dermis

Part I Research on the preparation of complex scalp cells suspensionObjetive:To investigate the efficient preparation of complex scalp cells suspension which contains epidermal cells and fibroblasts, and the acceptable ratio of complex scalp cells.Methods:1. Adult scalp digested with enzyme method to get complex cells.2. The cells were divided into 4 groups. Group A consisted of epidermal cells. Group B consisted of fibroblasts. Group C consisted of complex cells with the ratio of EC to FB 2:1. Group D consisted of complex cells with the ratio of EC to FB 6:1.3. Adult scalp digested efficiently with complex method to get complex cells.Rusults:1. Complex cells including epidermal cells and fibroblasts were successfully prepared by enzyme method.2. Cells in group C proliferated fastest, but there was a excessive proliferation of fibroblasts. The proliferation of cells in group D was slower than that of group B, while faster than that of group A, and there was a proper proliferation of epidermal cells.3. Complex method, compared with enzyme method, could evidently produce a more proper ratio of complex cells, faster cells proliferation, While there was no statistic differences in the cell totality, the cell viability and the ratio of cells expressing CD29 and K19 between the two methods.Conclusion:Complex method of digesting adult scalp, compared with enzyme method, could produce complex cells with less time, more proper ratio and faster proliferation. Partâ…¡Preliminary research on tissue engineering skins rapid construction by scalp cells combined with artificial dermisObjective:To investigate the effect of inoculating scalp cells into artificial dermis and test the proliferation of complex cells in many aspects.Methods:The primary complex cells were inoculated onto the surface of the artificial dermis to construct tissue engineering skins. The proliferation of the cells were evaluated by hematoxylin eosin staining, immunohistochemical staining and scanning electron microscopy.Results:After 6 days, the complex cells proliferated well in the artificial dermis. Different cells expressed PAN-CK, VIMENTIN, CD29 and K19 separately.Conclusion:The complex cells, inoculated into artificial dermis, cultured 6 days in vitro, showed proper proliferation. Tissue engineering skins were constructed preliminary.