The Study And Application Of Cyclodextrin In The Medication Analysis

Currently Cyclodextrin Chemistry has been developed into the most important subject of Supramolecular chemistry and is the pioneer of Supramolecular Chemistry compared with the macrocyclic compound such as crown ether, cave ether and so on. Cyclodextrin is widely used in areas such as agriculture, food, analytical technique and chemical industry. Cyclodextrin with the property of hydrophobic inside at the same time hydrophilic the outer can react with many objects (drug molecules, biological molecules, organic molecules and inorganic ions) to form inclusion compounds and the physical property, chemical property or spectral property of inclusion compounds will be changed. Therefore the inclusion mechanism between drug molecules and cyclodextrin can be studied via this principle. Also this principle can be directly tested for drug molecules. Besides indirect detection of drugs can be realized by construction of dye-cyclodextrin supramolecular system.In this paper, using molecule fluorescence spectroscopy and UV-Vis spectroscopy as research method, we have discussed the inclusion mechanism between dyes (fluorescein, neutral red), drug molecules (quercetin) and cyclodextrin. And also we have determined cholic acid with high sensibility and low detection limit. Simultaneously, we have discussed the reaction mechanism between quercetin and bovine serum albumin.There are five chapters in this paper.The first part reviews applications and progresses of cyclodextrin in the medication area.In the second chapter, the inclusion interaction between fluorescein (FL), neutral red (NR) and P-cyclodextrin have been studied. The binding constant K and stoichiometric ratio n were obtained respectively. The thermodynamic parametersΔG,ΔH,ΔS of inclusion compounds were calculated respectively. Also the differences between the two inclusion reactions were summarized.In the third part, a new, simple and highly sensitive P-cyclodextrin supramolecular system method for the determination of cholic acid has been developed. And we determined cholic acid with high sensibility and low detection limit based on the principle.In the fourth part, the inclusion interaction between quercetin and P-cyclodextrin has been studied using fluorescence spectroscopy. The binding constant K and stoichiometric ratio n were obtained. The thermodynamic parametersΔG,ΔH andΔS of inclusion compound were 3 calculated. The main action was confirmd by the value of hermodynamic parameters.In the fifth part, the interaction between quercetin and bovine serum albumin (BSA) was studied by the fluorescence spectroscopy and the UV-visible absorption spectroscopy. The static fluorescence quenching process of quercetin to BSA was confirmed. The binding constant and the number of binding sites were obtained.