The Experimental Research Of E-cadherin/Vimentin Protein Expression In The Bile Duct Epithelial Cells | Posted on:2011-10-02 | Degree:Master | Type:Thesis | Country:China | Candidate:M J Wang | Full Text:PDF | GTID:2154360308465630 | Subject:Hepatobiliary Surgery | Abstract/Summary: | PDF Full Text Request | Objective:Exploring the expression and importance of epithelial marker E-cadherhin and mesenehymal marker Vimentin in the bile duct epithelial cells which induced by LPS.Methods:We choose SD rats and the normal cells of human intrahepatic bile ducts epithelial as researchobject. Take the researchobject put into LPS(2ug/ml),we use immunohistochemical and Western Blotting technique in detecting both the markers of epithelial E-cadherin and mesenehymal Vimentin. Results:Animal experiments,We found E-cadherin gradually reduced (P<0.01) and Vimentin acquired masculine expression after induction (P<0.05). Cells experiments,the cell morphology of BEC happened mesenchymal after induction. By immunohistochemical detection we also found epithelial E-cadherin gradually reduced and Vimentin acquired masculine expression after induction (P<0.01). As to as Western Blotting results.Conclusion:E-cadherin gradually reduced and Vimentin acquired masculine expression in the bile duct epithelial cells after induction.That means epithelial-mesenehymal transitions had happened in the bile duct epithelial cells which inducted by LPS.
| Keywords/Search Tags: | intrahepatic biliary epithelialcells, LPS, E-cadherin, Vimentin, EMT | PDF Full Text Request | Related items |
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