| Objective Trigeminal somatosensory evoked potentials (TSEP) is formed by the nerve impulse stimulating the trigeminal nerve distribution area of the skin and mucous membrane receptors, we can record the potential arising from the trigeminal nerve branches in cortex, it can reflect in or around the branches of the trigeminal nerve lesions by recording the P1,N1,P2,N2,P3,N3,P4,N4,P5,N5 ten positive and negative waves. Blink reflex (BR) is one of the brainstem reflex, the technique is elicited electrically by delivering percutaneous stimulation over the supraorbital nerve, Responses are recorded over the right and left orbicularis oculi. We get R1 and ipsilateral R2 as well as contralateral R2'. Because both pathways are related with the trigeminal nerve, we selected thirty healthy volunteers and stimulated their supraorbital nerve, infraorbital nerve and mental nerve, comparing the TSEP latency differences of three branches,and evaluating the correction between TSEP and BR by stimulating supraorbital nerve,in order to establish the laboratory data to offer basic physiological index for clinical using.Methods We selected the 32 cases young volunteers, including 16 men and 16 women, aged 23-33 years old, The average age was 24.97±2.04 years old, One male has facial injury history in childhood, one woman has lower lip intermittent numb for pulling the mandibular opsigenes, the other 30 according to the following conditions:①No medically contributory select;②Without the trigeminal nerve and facial nerve injury history;③Without major diseases;④No surgical or traumatic history on skull or maxilla and mandible. The machine is DISA-2000M oculomusclar electromyogram made by Danmark, TSEP were recorded by stimulating three divisions of every healthy volunteers, and BR were also recorded at orbicularis oculi, then calculated the latency and amptitude of TSEP and BR.All data were dealt with statistical software SPSS 11.5 for windows by t test and q test, and the latency of waves of supraorbital nerve TSEP and BR were dealt with correlation analysis. There was a statistical significance when P< 0.05 occurred.Results1.The 30 healthy cases (60 sides) all obtained satisfied TSEP waves when stimulating the supraorbital nerve, infraorbital nerve and mental nerve, including the P1,N1 P5, N5 10 positive and negative waves; The BR were recorded in the orbicularis oculi muscle by stimulating supraorbital nerve, including three waves:The early ipsilateral response (R1) was single and sharpen.;The late ipsilateral response (R2) and late contralateral response (R2') were constituted by several waves, the latency were long and the form were complicated. The latencies of TSEP and BR were normal distribution, in statistic analysis we adopted t-test that the sex had not significant difference between male and female, it was the same for the left and right sides (P> 0.05).2.The variation between the TSEP of three branches, we adopted Analysis of Variance(ANOVA).The latencies of P1,N1, P2 of the various branches of the TSEP had statistically significant difference (P< 0.05), the latencies of N2,P3,N3,P4, N4,P5,N5 didn't have statistically significant difference, the latency of waves by stimulating supraorbital nerve were longer than the other two branches.3.The correlation of waves latency between TSEP and BR by stimulating supraorbital nerve was not significant.Conclusion The graphics of the TSEP are similar, this is in accordance with the nerve conduction pathway, and establish the laboratory data so as to offer basic physiological index for clinical using. As some pathway of TSEP and BR are identical, we should have a comprehensive analysis to determine neurological functional status in clinical. This method can provided some guidance for the positioning of patients with trigeminal neuralgia pain and the surgical site, and evaluate the postoperative effects. Both the two ways are non-invasive examination, simple, easy, and can provide objective quantitative indicators, so they are reliable electrophysiological inspection methods.
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