Preparation And Evaluation Of The Molecularly Imprinted Coatings For Capillary Electrochromatography

Molecularly imprinted polymer (MIP) is a new technique for synthesizing the stationary phase with the ability of predetermined selectivity. Capillary electrochromatography (CEC) is a new separation technique with high efficiency. The CEC-based MIP is the combination of the high efficiency of CEC and the high selectivity of MIP, which is a promising method.In the first part of this paper, in order to prepare MIP capillary coatings for a polar template, a new ternary porogen was developed (toluene-isooctane-DMSO). The d-zopiclone-imprinted coatings was synthesized by an in-situ thermo-initiated polymerization method and evaluated by CEC. The synthesis conditions and the CEC separation parameters that influenced the molecular recognition and chiral separation were studied systematically. The baseline separation of racemic zopiclone was obtained. Under the optimized conditions, the Rs value was 5.39 and the column efficiency of of-zopiclone was up to 21400 plates/m.In part two, one monomer (2-methacrylamidopopyl mathacrylate) molecularly imprinted polymer coatings were synthesized by an in-situ thermo-initiated polymerization method. On the resultant MIPs coatings, the column efficiency, the value of pH and the sample concentration were investigated. The effects of CEC parameters on EOF were also investigated. The baseline separation of racemic naproxen was achieved. Under the optimized conditions, the Rs value was 4.16 and the column efficiency of (S)-NAP was up to 13700 plates/m. Results suggested that one monomer molecularly imprinted polymer can effectively simplify the experimental design with higher selectivity and resolution than traditional molecularly imprinted polymer.