Effects Of Ginsenoside Rh2 On Tumor And Immune Indexes Of Colon Cancer CT26 Mice

Objective:To research effects of ginsenosideRh2 on tumor and immune index of colon cancer CT26 mice, searching for active ingredients of Chinese medicine which shows strong anti-tumor effect but less side effects.Methods:First of all, tumor cell lines were cultured and passaged. When the tumor cells reached a certain number, they were digested and off the sidewall with trypsin, later the tumor cells suspension were made by physiological saline, the tumor cells suspension was prepared。The tumor cells suspension was inoculated subcutaneously in 32 healthy BALB/c mice on the right neck and back. The mice were equal number of male and female. Two weeks after the inoculation, the tumor models were made and they were stable.24 of the mice of which the transplanted tumors subcutaneously were nearly the same size were randomly divided into three groups:ethanol ig group, ginsenosideRh2 ig group and 5-FU ip group.21 days after treatment, they were weighed and the data were recorded. Remove eyes of the mice, blood samples were gathered, centrifuged after standing, draw the upper Serum samples, Detect levels of serum IL-2 with Enzyme-linked immunosorbent assay. OD (absorbance) value of every serum IL-2 was measured at 450nm wavelength with Elisa Reader. Applied labsys Elisa Reader software v5.01 calculate every concentration of IL-2. Stripped mice subcutaneous tumor specimens, weighed by electronic balance and the data were recorded, preparing for calculating the inhibition rate of every group. And then sampled, fixed in 10% formalin, specimens were treated with immunohistochemical staining, detect the CD25+cell,Cleaved-caspase3 expression in the subcutaneous tumor in mice with inverted microscope. Removed the thymus, spleen of every mice, weighed and the data were recorded, after then calculated thymus index, spleen index.Results:1. Compared with ethanol ig group, the inhibition rate of ginsenosideRh2 ig group is 81.76%. Compared with ethanol ig group, the inhibition rate of 5-FU ip group is 90.32%.2. The effect of ginsenosideRh2 on Cleaved-caspase3 expression in the subcutaneous tumor in mice:Compared with ethanol ig group, the Cleaved-caspase3 expression rate in the subcutaneous tumor of ginsenosideRh2 ig group Significantly increased, shows statistically significance.3 Compared with 5-FU ip group, the CD25+ cell expression rate in the subcutaneous tumor of ginsenosideRh2 ig group is less than the former.4. Compared with ginsenosideRh2 ig group and ethanol ig group, the levels of serum IL-2, thymus index and spleen index of the 5-FU ip group decreased.5. The effect of ginsenosideRh2 on immune organs of tumor bearing mice: Compared with ethanol ig group, thymus index and spleen index of the ginsenosideRh2 ig group shows no statistically significance.6. The effect of ginsenosideRh2 on Cytokines of tumor bearing mice:Compared with ethanol ig group, the levels of serum IL-2 of ginsenosideRh2 ig group shows no statistically significance.7. The effect of ginsenosideRh2 on CD25+ cell expression rate in the subcutaneous tumor in mice:Compared with ethanol ig group, the CD25+ cell expression rate in the subcutaneous tumor of ginsenosideRh2 ig group shows no statistically significance.8. Compared with the intact mice, the levels of serum IL-2, the thymus index and spleen index of mice decreased after bearing tumor.Conclusion:GinsenosideRh2 can inhibit the growth of colon CT26 tumor through ig 0.4mg/kg. Increase the Cleaved-caspase3 expression rate in the subcutaneous tumor. Promote the apoptosis of colon CT26 tumor. But it is no better than 5-FU. But in terms of side effects, it is better than 5-FU.And no Significant effect on increasing the levels of serum IL-2, the thymus index and spleen index of the mice bearing tumor.