Primary Study Of Fu-Fang-Chang-Tai On The Inhibition To Colon Cancer

Colorectal cancer is one of the most common cancers in both men and women.To observe the effects of Fu-Fang-Chang-Tai(FFCT) on the growth of SW480 human colon cancer xenografted in athymic mice,and on cell growth,cell generation cycle and apoptosis of human colon carcinoma cell SW480,and on the immune function of mice with carcinoma through in vivo animal experiments and in vitro cell experiments.The mechanisms were explored at levels from whole animal and histological to cytobiological and molecule-biological.The methods were used as follows:The best one was sieved from several prescriptions of FFCT through the experimental effects on the growth of human colon cancer cells SW480 and SW480 xenografted in athymic mice.The effects of different saturation of FFCT in different action time on cell multiplication of SW480 in vitro culture were determined with methyl thiazolyl tetrazolium(MTT) chromatometry.At the same time the changes of cell generation cycle and apoptosis rate were detected with flow cytometry.Caspase-3 enzyme activities in FFCT-treated SW480 cells were determined. The comparison was made between apoptosis rates in FFCT-treated cells and Ac-DEVD-CHO with FFCT-treated cells.The effects of FFCT acting on the tumors, immune organ weight and the formation of hemolysin antibody of the mice with hepatocarcinoma H22 were studied.The results came out as follows:The growth of human colon carcinoma cell SW480 and SW480 xenografted in athymic mice could be significantly inhibited by 5^th FFCT, while the body weights of the mice treated by 5^th FFCT were not light which compared with the control group.The effectiveness of 5^th FFCT was better than other prescriptions. The generation of colon carcinoma cell SW480 in the stage of G₀/G₁ and G₂/M could be interrupted by FFCT.To some extent,the more saturation and time of FFCT acted,the more of inhibitory action on tumor cell growth and apoptosis rate showed.Caspase-3 enzyme activity in FFCT-treated SW480 cells increased at first and then lysised with time went compared with the control group.Both apoptosis rates and death rates in Ac-DEVD-CHO with FFCT-treated cells increased significantly compared with the control group.Apoptosis rates in Ac-DEVD-CHO-with FFCT-treated groups were lower than in corresponding FFCT-treated groups while death rates were similar with them. There were some synergistic actions in Cyclophosphamide united with FFCT to inhibit tumor growth.It could improve the situation of organ atrophy and low formation of hemolysin antibody of the tumor-beating mice caused by chemotherapy in that organism immunity.The humoral immunity could be improved.The conclusion indicated that:Colon carcinoma could be cured by FFCT through the growth of tumor weight inhibition and the immune function improvement.The growth cycle of tumer cells could be interrupted and cell apoptosis could be induced to inhibit the growth of human colon carcinoma cell SW480 on the effect of FFCT.Caspase-3 way was an important but not the only way in the process of SW480 apoptosis.