The Experimental Study On OVA-induced Pulmonary Pathological Changes By Transfering Dendritic Cells Of Schistosoma Japonicum-infected Mice

Objective This paper aimed at studying the effect of dendritic cells (DCs), which were adoptively transferred from Schistosoma japonicum-infected mice, on the expression of CCL-11 and IL-13Ra2 and the infiltration of EOS induced by OVA in lung tissue. Methods DCs of Schistosoma japonicum-infected mice and naive mice were respectively isolated and purified using CD11c+ microbeads.30 female BALB/ c mice (7-week old) were randomly divided into 6 groups (5 mice/group).The mice in Group A were naive mice. The mice in Group B were OVA-treated only. Group C included Group C1 and Group C2.The mice in Group C1 were OVA-treated after adoptive transfer of DCs of Schistosoma japonicum-infected mice (SJDC/OVA).The mice in Group C2 were OVA-treated after adoptive transfer of DCs of naive mice (NDC/OVA).Group D included Group D1 and Group D2.The mice in Group D1 were adoptively transferred with DCs of Schistosoma japonicum-infected mice after OVA treatment(OVA/SJDC).The mice in Group D2 were adoptively transferred with DCs of naive mice after OVA treatment (OVA/NDC). The mice in Group D were sensitized with OVA.3 weeks after sensitization, purified CD11c+ cells were first washed in protein-free PBS,and then injected into the tail vein of the mice in Group C1,C2, D1 and D2(1×106/mouse). The mice in Group C1 and D1 received DCs of Schistosoma japonicum-infected mice, while the mice in Group C2 and D2 received DCs of naive mice.30 minutes later, the mice in Group D were challenged intranasally with 50μg OVA for 7 consecutive days, and then the mice were killed and their lungs were collected for further analysis.2 hours following adoptive transfer, the mice in Group B, C1 and C2 were sensitized and challenged with OVA in the same manner.24 hours following the last challenge, the mice in Group A, B,C1 and C2 were sacrificed, and their lungs werecollected. The lungs of the mice in all six groups would be used for histological and immunohistochemical analysis. Results Histological analysis showed that pulmonary inflammation of the mice in Group C1 and D1 diminished markedly, compared with the mice in Group B,C2 and D2(P< 0.05).Immunohistochemical analysis showed that, for the mice in Group A, B,C1,C2, D1 and D2,the percentage of the positive area of CCL-11 in the blood vessels and tracheas in lung tissue were successively 0.1653±0.0214,0.5339±0.0322, 0.2737±0.0218,0.4818±0.0199,0.2397±0.0239 and 0.4857±0.0141;The average optical density (AOD) of the positive area of CCL-11 in the blood vessels and tracheas in lung tissue were successively 0.1674±0.0104, 0.3874±0.0236,0.3160±0.0235,0.3082±0.0197,0.2546±0.0172 and 0.2398±0.0121; the percentage of the positive area of IL-13Ra2 in the blood vessels and tracheas in lung tissue were successively 0.1722±0.0108,0.5440±0.0281,0.2543±0.0341, 0.4470±0.0330,0.4994±0.0304 and 0.3051±0.0198;AOD of the positive area of IL-13Ra2 in the blood vessels and tracheas in lung tissue were successively 0.1746±0.0090,0.4014±0.0130,0.3266±0.0144,0.4228±0.0058,0.2808±0.0102 and 0.2566±0.0093.Compared with the mice in Group B and C2, AOD and the percentage of the positive area of CCL-11 and IL-13Ra2 of the mice in Group C1 significantly decreased(P<0.05);Compared with the mice in Group B and D2, AOD and the percentage of the positive area of CCL-11 and IL-13Ra2 of the mice in Group D1 reduced markedly(P<0.05).Conclusion DCs of Schistosoma japonicum-infected mice played a critical role in the inhibition of OVA-induced allergic asthma, which indicating their preventive and therapeutic functions on asthma.