Correlative Study Of Advanced Glycation Endproducts (AGEs) In The Serum And Periodontitis Severity Of Periodontitis Rat With Diabetes

Objective:The goal of this study was to establish a diabetic periodontitis model and to observe the changes of Advanced Glycation End Products(AGEs) in periodontitis rat with diabetes for the study of the relationship between AGEs and diabetes.Methods:40,200g,2 months old Wistar male rats were randomly divided into four groups,the control group (N=10), periodontitis group (P=10), diabetic group (DM =10),diabetic periodontitis group (DP=10).The DM group was established by intraperitoneal injection of streptozotocin(STZ).The P group was established by ligating floss around maxillary frist molars.with orthodontic wire ligature wire. The DP group was established by intraperitoneal injection of STZ and by ligating floss around maxillary frist molars with orthodontic wire ligature wire. Body weight change, food water intake, urine output, activity, coat color were observed.The blood sugar, the pocket depth,the periodontal bone loss were be measured.Four and six weeks later, animals were sacrificed and the specimens were prepared for pathological analysis.Serum AGEs were measured by ELISA method. Observed pathological changes of periodontal tissue was for correlation analysis of serum AGEs and periodontitis.Significant differences between groups were evaluated by One-way ANOVA,LSD-t test, paired t-test, person correlation.Results:The polydipsia, polyphagia, polyuria, weight loss were observed three days after injection of STZ citrate buffer in the DM group.Lively, well-proportioned body, responsive, less drinking water per day, less urine output, smooth coat, body weight increased steadily with increasing weeks of age were observed in N group rats. Food impaction, saliva more, periodontal pocket formation, alveolar bone loss and other symptoms were also observed in P group.The polydipsia, polyphagia, polyuria, weight loss were observed three days after injection of STZ citrate buffer in the DP groupand.Food impaction, saliva more, periodontal pocket depth formation, alveolar bone loss and other symptoms were also observed in DP group.4 week and 6 week later, periodontal pocket probing depth and periodontal bone loss were serious of 6 weeks compared to 4 weeks in DP group DM group.Blood glucose was significantly higher than N group and P group,the difference was significant (P<0.05).four groups of rats probing depth DP group and DM group,DP group and P group, DP group and N group, P group and the DM group, P group and N group there were significant differences between (P<0.05).Gingival hyperplasia, periodontal pocket formation, papilla trench epithelial ulcers, necrosis.Alveolar ridge to severe alveolar bone osteoporosis, osteoclasts can be seen in the DP group and P group.DM group N was no more favorable gingival epithelial integrity, surface marked hyperkeratosis, epithelial spikes more than the slender, non-keratinized sulcular epithelium without spikes, combined with epithelial adhesion in the cemento enamel junction Department, alveolar crest height normal.4 weeks,6 weeks later DP group and N group, DP group and P group, DM group and P group, DM group compared with the N group differences in serum AGEs was significantly (P<0.05).That periodontitis periodontal pocket probing depth in DP group and the concentration of serum AGEs correlated in the group of diabetic rats (r=0.846, P<0.05)Conclusion:1.The methods can be successfully produced in rat model of each group.2.Group of diabetic periodontitis rats was positively correlated with the concentration of serum AGEs and probing depth. Serum AGEs were significantly associated with deterioration of periodontitis.3.The high levels of serum AGEs might be a risk factor for periodontitis.