The Establishment Of Smoking Rat Animal Model Of Experimental Periodontitis

Subjective:To establish experimental animal model of periodontitis on smoking rats.Methods:Forty health Wistar rats were randomly divided into four groups as follow:control group(C),ligation group(L),smoking group(S),ligation and smoking group(SL).Every group was ten.The control group was raised with no treatment.The ligation group was ligated by two erthodontics wires around the cervical of the second molar.The smoking group was subjected to passive smoking box induce to smoke 12 cigarattes every time for half an hour,twice one day.The last group,ligated as the ligation one,smoks together with the smoking one every day.Observe the inflamation of periodontal tissue every week. PD becomes deeper,recording the GI,PB and the bone loss through clinal exam,X-ray and historigical exam.At the same time,IL-6 was determined in the serum and gingival tissue.Results:6 weeks later,PD got deeper in SL group.Except PD and the IL-6 content in serum were significantly higher than C,S group.The indexs of SL group were higher. while IL-6 content in serum in S was h.At the 8th week,PD got deeper in L group.every index,except IL-6 content in serum,were higher in L and SL group than their contrast ones.The GI made no difference between the L group and the SL one(p>0.05).IL-6 in serum in the S or SL was still higher(p<0.05).The C group contained.Conclusion:1 Both passive smoking and ligation that given on rat can successfully establish animal model of periodontitis.2 Six weeks later,the experience animal model of periodontitis on smoking rats is successful.