Correlation Analysis On Brain Leptin In Young Rat With Metabolic Syndrome

Objective:To investigate the effects of food high in fat and salt on hypothalamic leptin, body fat distribution,blood pressure, glucolipid metabolization and the correlation between hypothalamic leptin and other indices,then evaluate the mechanisms of leptin on metabolic syndrome.The result will provide us with new methods on treatment and prevention of metabolic syndrome.Methods:Forty young SD rats,half males and half females,were randomly divided into three groups:high fat and high salt group(14 cases),high fat group(14 cases) and control group(12 cases).After four weeks,OGTT,hypothalamic leptin,blood pressure,blood lipid, cortisol, body fat distribution,blood glucose and insulin were measured.Results:1.In high fat and high salt group,body weight,waist circumference and visceral fat weight were significantly higher than in control group (P<0.05);In high fat group,body weight and visceral fat weight were significantly higher than in control group(P<0.05).2.In high fat and high salt group,blood glucose and serum insulin at fasting,60 min,120 min and 180 min were significantly higher than in control group(P<0.05).In high fat group,blood glucose at 60 min was significantly higher than in control group.3.In high fat and high salt group,systolic pressure,TC,TQLDL-C were significantly higher than in control group (P<0.05),while HDL-C were significantly lower than control group(P<0.05).4. In high fat and high salt group brain leptin were significantly lower than in high fat group and control group(P<0.05).In high fat group brain leptin was significantly lower than in control group.5.In high fat and high salt group brain leptin levels were negatively correlated with visceral fat weight(r=-0.453),fasting blood glucoseserum(r=-0.286),insulin (r=-0.566),systolic pressure(r=-0.472) and TG (r=-0.511),while positively correlated with HDL-C(r=0.384).In high fat group,brain leptin levels were negatively correlated with visceral fat weight(r=-0.179),insulin(r=-0.464),blood pressure(r=-0.286) and TG(r=-0.607),while positively correlated with HDL-C(r=0.643).Conclusion:1.Model of metabolic syndrome was made via food high in fat and salt.2.Brain leptin levels were negatively correlated with visceral fat weight,fasting blood glucoseserum,insulin, systolic pressure and TG, while positively correlated with HDL-C.Metabolic syndrome is the result affected by many fators.3.Brain leptin may play a role in metabolic syndrome via affecting body fat distribution,blood pressure and glucolipid metabolization.