Expression And Clinical Significance Of GLUT1 In Clear Cell Renal Cell Carcinoma

Objective and Background:The morbility of renal cell carcinoma is next to bladder tumor in the malignant tumor of urinary system in china, about 2%-3% in adult malignant tumor. There have a few reports on the correlation between the expression of GLUT1 gene and clear cell renal cell carcinoma. This study was to discovery the expression and significance of GLUT1 gene in both renal cell carcinoma (RCC) and the normal renal tissue. We would analyzed the relationship between GULT1 gene and development, progress, clinical stage, and pathological grade of renal cell carcinoma, willing to provide new clinical theory basis for the diagnosis and therapy of renal cell carcinoma.Material and Methods:This study included 50 cases of patients, from October,2006 to June,2008, who admitted in the General Hospital of Tianjin Medical University for the treatment of RCC. There are 39 male and 11 female. The median age was 59 (range 41-81). The clinnical stage was assigned according to the 2002 AJCC TNM system:11 patients of T1,3 patients of T2, and 36 patients of T3. For pathologic grading:15 patiens of GradeⅠ,25 patients of GradeⅡand 10 patients of GradeⅢ. None of them had received chemotherapy or radiotherapy before operations.10 specimens of normal renal tissues were acquired as control. Immunohistochemical staining for GLUT1 was carried out and SPSS13.0 for windows was used to evaluate the association between GLUT1 expression and development, progress, clini co-pathologic parameters.Result:1. The positive rate of GLUT1 expression in CCRCC was 100%. The positive degree were moderately or strong. While none of the GLUT1 expression in normal renal tissue was found. GLUT1 expression was significant differential between CCRCC and normal renal tissues (P<0.01).2. The different degree of GLUT1 expression was related to age. The strong positive rate of GLUT1 expression in younger (100%) is more than that in more than 50 years old patients (54.8%). A statistically significant difference was observed between them (P< 0.05). No statistically difference of the different degree of GLUT1 expression was seen in sex and the size of tumor (P>0.05).3. The expression rates of GLUT1 in the moderately positive group were 21.1% (4/19),5.2%(1/19) and 73.7%(14/19) in T1,T2,T3, respectively. While in the strong positive group were 22.6%(7/31),6.4%(2/31) and 71.0%(22/31), respectively. No statistically difference in two groups(χ2=0.052,P=0.97>0.05). Although the positive expression of GLUT1 in T2+T3 group (78%) were higher than that in group T1 (22%), no statistically difference in the two groups (χ2=0.016,P=0.90>0.05).4. The expression rates of GLUT1 in the moderately positive group were 31.6% (6/19),57.9%(11/19) and 10.5%(2/19) in G1,G2 and G3, respectively. While in the strong positive group were 29.0% (9/31),45.2%(14/31) and 25.8%(8/31), respectively. No statistically difference in two groups (χ2=1.783, P=0.410>0.05). Although the positive expression of GLUT1 in G2+G3 group (70%) were higher than that in group G1 (30%), no statistically difference in the two groups (χ2=0.018, P=0.90 >0.05). Conclusion:1. The study had shown that there was more expression of GLUT1 gene in CCRCC than in the normal tissue. A statistically significant association was observed between them.2. The expression of GLUT1 gene in CCRCC was positive or strong, which give us a hint that the intensive expression of GLUT1 gene was related to the development and progress of CCRCC. GLUT1-targeted therapy may be a promising strategy to prevent progression of CCRCC.3. The positive degree of GLUT1 expression gene in CCRCC was related to age. The expression of GLUT1 gene was more in the younger patients. Maybe stronger metabolic capability of tissue in younger patients.