Study On The Lecithin For Fat-dissolving

BackgroundWith the growth of age, fat accumulation, facial fat shift, fat atrophy, which are caused by a variety of reasons, become important factors in facial aging. There are many operations that can improve the adipose tissue deformation, liposuction to eliminate fat accumulation; Radiofrequency heat shift of fat(instance:baggy eyelids plasty); Facial depression filled with autologous fat and the emerging "Mesotherapy" for fat-dissolving--the use of drugs injected into the fatty layer.A variety of methods for the operation of the fat different issues have been applied to achieve the goal of being young again. Traditional liposuction and the Mesotherapy imported from abroad, have become the two most important methods in weight-loss, and attracted all of the domestic doctors of plastic surgery because of their respective characteristics.How to choose the different approaches to carve fat has become an emerging issue that need to be solved by cosmetic plastic surgeon. With the introduction of the term of "Mesotherapy" in domestic academic and the gradually increasing understanding of the term, lots of questions without definitive answers have raised, such as how to translate "Mesotherapy"; the safety and validity of Mesotherapy;whether the dissolving fat method of medical equipment and chemicals used in synthetic drugs need to be verified by medical-related institution. Perfect Body shaping is an important component of cosmetic surgery, in this field liposuction technique is a clinical use of minimally invasive method to lose weight and has been widely applied. Recently a new method, Mesotherapy, imported from abroad used into fat-dissolving has offered us another way which makes it possible to engrave on the body using non-invasive method in order to achieve good results. The security of the application of the liposuction technique is sure, while the security of the application of Mesotherapy is worth exploring. The lecithin is widely used as an experienced therapy in foreign countries, improving local fat distribution by injection with fewer clinical complications. Lecithin for fat-dissolving is the key in the mesotherapy, so it is the research object in my study.Nowadays, some drugs breast augmentation have been widely used in injection for breast augmentation, a large number of cases have proven they do not have the security; the number of mesotherapy on the foreign reports is increasing; Injection of fat soluble lecithin has been organized in domestic classes continuously by some foreign experts. At the same time, some medical plastic hospitals and beauty parlours are introducing various foreign injection of drugs to make huge profits; in the eyes of domestic consumers consumption of plastic surgery has become firmly rooted, not only women but also men began to start a bloodless non-surgical cosmetic injections rush. To sum up, the safety of the lecithin for fat-dissolving, it's taken for granted as we orthopedic doctors eager to study.Lecithin also known as soy lecithin, or phosphatidycholine(PC) is a white waxy material which is usually solid, easy to absorb moisture, but is insoluble in water. Phosphatidylcholine is closely related with the metabolism of fat. In September 1996 in the seventh international conference on lecithin held in Brussels, medicine and nutrition experts have confirmed that lecithin (soybean phospholipid) as a nutrition, played an important role in improving health and preventing disease. It has already won the world nutrition experts, pharmaceutical scientists and physicians of the generally recognized. Lecithin as a functional health food does not show immediate results, but show a comprehensive, long-term, stable effect without side effects of drugs. Therefore, physicians are interested in its characteristics, and a large number of studies have shown that their following diseases have a very good role in prevention.Deoxycholic acid (DC), on the lack of a C-7-hydroxy bile acids, loss of oxygen atom is derived from bile acid derived from a kind of free bile acid, mainly in the bile of taurine, glycine binding form by acid oxidation. DC has the promotion of bile secretion of anti-inflammatory effects. DC, as a lecithin solvent, makes PC easy to injection of water-soluble substance and organization to absorb.Objective:To make a preliminary study on the effects, safety, medicines specificity and combinations of lecithin during its introduction for fat dissolving in clinic applications.Methods:Experiment 1:Two different kinds of animals were selected:10 New Zealand white rabbits and 10 Kunming mice of 10. The fat of those animals concentrates behind the neck and groin. Then subcutaneous injection of lecithin was prepared in the fat appearance, the effects of different animal fats on the same effectiveness of a drug. New Zealand white rabbits and Kunming mice of 10, the site of fat distribution concentrated behind the neck and groin, respectively fatExperiment 2:Lecithin Lecithin (PC) injection (in order to deoxycholic acid (DC) as the solvent) from Beijing Tong Zhi-lin Biotechnology Development Co., Ltd products. Animal groups:40-day-old female Kunming mice,40 (by the Guangdong Medical Experimental Animal Center), weight (22.080±3.032) g Sub-boxes using mouse-specific feed, feeding, each group of Kunming mice drinking water equivalent control. Animals were randomly divided into two groups of 20:①the experimental group,②blank control group.Experimental specific methods:the experimental group:Every once 5d injection. In the experimental group completed five courses upon completion of injection of concentrated death, compared to observation the fat mass. Drawn Methods:the unilateral conduct of fat at the groin side with drugs of the mice was all removed, cleaned in the saline wash to be weighed. Inguinal fat weight weighing, accuracy of 0.001g.Each specimen was cut, and then observed under high power microscope to detent the microstructure change of various organs (liver, kidney, skeletal muscle and adipose tissue) derived for staining tissue sections. And actions about observation of mice eating were carried out.Experiment 3:40 40-day-old female Kunming mice(from the Department of Basic Medicine, Harbin Medical University Experimental Animal Center), were randomly divided into two groups of 20:①Lecithin experimental group②Deoxycholate experimental group. Injection of phosphatidylcholine and deoxycholate was applied to the two groups; Experimental Methods were the same as those in experiment 2.Results:Experiment 1:Based on the visual observation point of view, phosphoric acid lecithin of fat in two different animals has clear dissolved. Both X-shaped fat in the New Zealand rabbit's neck and the fat in the groin of Kunming mice were significantly reduced on the volume.Experiment 2:Dissolution of lecithin on the fat than the obvious, but the lack of apparent specificity. Visual observation:subcutaneous injection of subcutaneous fat than the group without injection of the blank side of adipose tissue decreased significantly. Muscle tissue color and slightly dim, loss of normal luster. Histological observation:very few muscle fibers dissolved fracture(Figure 2-1). Fat cells of the cell wall permeability change, Lipid droplets overflow cells a series of changes:The cell wall thickness of different parts was different, rupture of fat cells dissolve into each other in size, irregularly shaped vacuoles. Inside and outside the vacuoles, there are several kinds of cells:fibroblasts, lipid droplets engulfed by macrophages and epithelial-like cell proliferation, as well as monocytes and lymphocytes infiltration (Figure 2-2). The disappearance of organizational change of the degeneration of water and organizational congestion occurring in the liver, kidney.Experiment 3:Lecithin group mainly as a result as follows:Lecithin, and deoxycholic acid both have the dissolution of the destructive effects of muscle fiber, figure 3-1 and Figure 3-4;as well as the role of lipid metabolism, figure 3-2,3-3,3-5 and 3-6. Vacuolization exists in liver and kidney. The Deoxycholate-breaking will be the role of more serious, table 3-1.Conclusion:Lecithin dissolved in the promotion of fat does play a role. Fat cells are dissolved at the cell walls, the cells wall thickness of the same cells is uneven, and in broken cells, the mutual dissolution of size, shape irregular vacuoles form. Pure injection of lecithin and deoxycholate are destructive on the liver, kidney and muscle tissue, and either has the effect on both of the lipid metabolism and muscle tissue. After a certain percentage of the ratio, scientific formula has a smaller damage for tissue.Lecithin for fat-dissolving is not strict tissue-specific, damaged the muscles, liver and kidney. Although the proportion of different drugs on the tissue damage in varying degrees, it is recommended that clinicians be cautious to operate. In particular, strictly control the scope of application of lecithin:mid-level amount of fat injections into the middle of plate-like fat Because of dissolution of the muscles, it's not suitable for the face in which there are more complex distribution of neuromuscular facial blood vessels, applies only to the middle plate-like fat injections. There is terrible damage to the liver and kidney, so it should not applied at excessive doses, and it should be used in a small amount of repeated injections, giving the body a metabolic process in order to reflect the absorption of fat soluble lecithin another injury in the small features.