Observation Of Curative Effect Of QingChangHuaShiMethod Combined With Enema Drug Treating Active Ulcerative Colitis And Experimental Study On Some Specific Chinese Medicine

Objective The aim of this study is to observe the clinical effect of QingChangHuaShi method combined with enema drugs to treat active ulcerative colitis and also to evaluate the safety of this method; to observe the effect of specific traditional Chinese medicine in these prescriptions such as Phellodendn,Sanguisorba,Bletilla,Japanese Ampelopsis Root to explore the possible action mechanism of traditional Chinese medicine and to screen a development durg through animal experiment.Methods Clinical research:Sixty ulcerative colitis patients of damp-heat syndrome were randomly divided into two groups:treatment group and control group (30 cases in each).The patients in treatment group were given QingChangHuaShi Prescription orally in combination with a Chinese herbal enema, while the patients in control group were treated with mesalazine (5-ASA).After 3 months treatment, we did a clinical effect assessment between the two groups.Experimental study:Seventy SD rats were randomized divided into seven groups as follows:normal control group, model control group, Phellodendron treatment group, Sanguisorba treatment group, Bletilla treatment group, Japanese Ampelopsis Root treatment group and Balsalazide (5-ASA) treatment group.The rat model of ulcerative colitis was induced by TNBS.Each treatment group was administered by gastric infusion for ten days after model-making. Then we observed the pathological changes of colonic mucosa by HE stain and detected the expression of TNF-α,IL-1α,IL-10 in the rat's serum and NF-κB p65 protein of the rat's colon tissue sepreately by the method of ELISA and Western blot.Results Clinical research:The effect of improving symptoms of diarrhea, mucous bloody stool, abdominal pain, distension, tenesmusin treatment group was better than that in control group (P<0.05),but there was no statistical difference between the effect of improving anal burning of the two groups (P>0.05).There was statistical difference between the total effective rate of the two groups, and the curative effect in treatment group was better than that in control group (P<0.05).The effect of improving the lesions of colonic mucosa in treatment group was better than that in control group (P<0.05). The effect of improving the remission rate in treatment group was better than that in control group (P<0.05).Experimental results:All of the treatment groups can improve the colonic macroscopic morphous and pathohistology of rats (P<0.05).Compared to model control group, the expression of TNF-α,IL-1βin the rat's serum was lower and the content of IL-10 was higher, the expression of NF-κB p65 protein in colonic tissue in each treatment group was less than that in model group (P<0.05).Among these treatment groups,the expression of NF-κB p65 protein in Sanguisorba prescription group was significantly lower(P<0.05).Conclusion QingChangHuaShi method was effective and safety in treating ulcerative colitis of damp-heat syndrome.According to the experimental study, we conjecture that the mechanism of Phellodendron,Sanguisorba may be restraining inflammation and regulating immune through the expression of IL-1β,IL-10,TNF-a and NF-κB p65 protein; Bletilla and Japanese Ampelopsis Root played a therapeutic role may be through other ways.