| Ulcerative Colitis (UC) is a chronic and relapsing disease of the large intestine (rectum and colon) characterized by inflammation of the mucosa and submucosa. This study compared the therapeutic effect and inner anti-inflammation mechanism of treating UC with Kui Jieyin in two kind of medications (peroral & enema).In the experiment , the rats were randomly divided into 5 groups: normal group, model group, Olsalazine sodium group( the controlled group), ig KJY group (Kui Jieyin peroral) , in KJY group (Kui Jieyin enema) and the last 4 groups are UC rats induced by 2,4,6-trinitrobenzenesulfonic Acid (TNBS) /ethanol mixture. We took serum and colon tissues from 1/3 of the animals every time after giving medicine for lweek, 2 weeks and 4weeks and compared the effect of the two medications as the following aspects:1. The different effect on the UC rats(1)Comparison of the effects on living state of the rats, the general behaviors and the weight involved.(2)Comparison of the local pathological change, both gross and microscopic observations included and counting the scores of the inflammation-degree .2. The different anti-inflammation mechanisms due to the different medications (l)The inhibition of the expressions of pro-inflammation cytokines witch can facilitate the inflammation and are related with relapse of UC: To determine the contents of tumor necrosis factor (TNF)-a, IL-8 in serum and observe the expression of TNF-a in local colon mucosa(2)The effects on the expression of nitric oxide (NO) and iNOS: To determine the content of NO in serum and observe the expression of iNOS in local colon mucosa.We can draw the following conclusions according to the results: (l)The variance of the effect on the UC rats due to the two medications:On the aspect of improving the living state, Kui Jieyin peroral or enema can both have obvious effect since the acute period. Kui Jieyin enema has better effect than Kui Jieyin peroral and its effect can last to the chronic period. The long-term effect of Kui Jieyin enema is more available.As the observation of the local colon mucosa, both Kui Jieyin peroral and enema have the effects of inhibiting the injury and and improving the plerosis of mucosa tissues. The effect emphasized on the different aspect. (2)The comparison of the anti-inflammation therapeutic mechanism of the two medications:As the inhibition of the expression in serum of cytokines which canfacilitate the inflammation and are related to relapse, Kui Jieyin peroral is better than enema in the earlier stage (the 1st week) of the acute period. In the later stage of the acute period (the 2nd week), Kui Jieyin enema is similar to peroal in decreasing TNF-a and better than peroal in decreasing IL-8. Kui Jieyin enema shows the effect later than peroal, and both peroral and enema have no significant effect in the chronic period.As the effect on the expression of TNF-a in local colon mucosa: Througut the acute and chronic period, 3 therapies can all decrease the expression of TNF-a in mucosa. Kui Jieyin enema and Olsalazine sodium are both better than Kui Jieyin peroral in the acute period. There's no significant difference among all the therapy groups in the chronic period.As the accommodation on the secretion of NO, Kui Jieyin enema can both increase the secretion of NO protecting the mucosa and inhibit the secretion of NO injurying the mucosa in the acute period. This role is bidirectional and similar to Olsalazine sodium. Kui Jieyin peroral has no regulation effect.As the effect of the expression of iNOS in local colon mucosa, all the therapies have the effect of increasing the expression of iNOS in mucosa during the earlier stage of the acute period. And all the therapies have the effect of decreasing the expression of iNOS in mucosa during the later stage of the acute period. Kui Jieyin enema is better than Kui Jieyin peroral and Olsalazine sodium the whole period. In the chronic period, all the therapies don't have the effect of accommodating the expression of iNOS in colon mucosa. |
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