Proteomics Technology Compare The Differential Expression Proteins In Perilesional Brain Tissue Between Cerebral Hemorrhage 6 Hours And 72 Hours

Objective: Intracerebral hemorrhage(ICH) refers to internal hemorrhage of brain substance caused by brain arteries,veins or capillaries rupture. Cerebral hemorrhage accounted for 20%～30%of acute cerebrovascular disease. Morbidity and mortality rates is high, it seriously endangers people's health . At home and abroad,ICH is attached great importance to. Edema appeared after cerebral hemorrhage 6h and reached the peak about 72 hours ,then gradually subsided after two weeks. Cerebral edema increased brain tissue injury. The key of reducing mortality and disability rate is to control brain edema and intracranial hypertension. If you can clear that differential expressed proteins in perilesional brain tissue between cerebral hemorrhage 6 hours and 72 hours, it is significant for right understanding the pathological mechanisms of cerebral hemorrhage and edema after ICH in molecular level and the development of new drugs.The proteomics is a key area of research that is developing in the post-genome era.Proteomics is an ideal instrument that can compare protein level between different samples. Proteomics offers scientists the possibility of identifying disease-associated protein markers to assist in diagnosis or prognosis and to select potential targets for specific drug therapy.In this study, by proteomics technologies we dynamically study perilesional brain tissue of intracerebral hemorrhage animal models. Comparing the differential expression proteins in perilesional brain tissue between intracerebral hemorrhage 6 hours and 72 hours is aimed to understand the molecular mechanism of cerebral hemorrhage and edema after ICH, and provides an experimental basis for searching for more effective treatment approach . Methods: The stereotactic apparatus were used making rat animal models of the caudate nuclei hemorrhage . The perilesional brain tissue were took as samples after 6 hours and 72 hours. Brain tissue protein were extracted to carry out two-dimensional gel electrophoresis: immobilized PH gradient (IPG) isoelectric focusing electrophoresis was run as the first dimension, and vertical SDS-PAGE as the second dimension .The maps were visualized by colloidal coomassive blue R250 and analysise dwith ImageMaster 2D Elite software .The proteins of interest were digested and identified using MALDI-TOF mass spectrometry.Results: The expression of 41 proteins are significantly different between the 6h group and the72h group after cerebral hemorrhage.19 up-regulated proteins of 72 hours after intracerebral hemorrhage identified as Dihydrolipoyllysine-residue acetyltransferase component pyruvate dehydrogenase complex, Vacuolar ATP synthase submit Brain isoform, Glial fibrillary acidic protein, Actin-related protein3,Cytosol aminopeptidase, Phosphoglycerate kinase1,Elongation factor tu,mitochondrial, Septin-5 , Purine nucleoside phosphorylase, Prohibitin, Annexin-A5,14-3-3protein-ε, F-actin-capping protein submitα2,β-synuclein, Protein-L-isoaspartate(D-aspartate)O-methyltransferase, Adenylate kinase isoenzyme 1, Pyruvate kinase isozymesM1/M2, Mitogen-activated protein kinase 1, T-complex protein submitε. 21 down-regulated proteins in 72h group after ICH identified as Transitional endoplasmic reticulum ATPase, endoplasmin , Fascin(fragments), Dihydrolipoyl dehydrogenase mitochondrial, Pyridoxal kinase, Voltage-dependent anion-selective channel Protein 1, Carbonic anhydrase2 , Phosphoglycerate mutase 1, Glutathione S-transferase Yb-3, Transgelin-3, Adenosylhomocysteinase, Dual specificity mitogen-activated protein Kinase kinase 1, Dihydropyrimidinase-related protein5, Vacuolar proton pump submit E1, Dihydropteridine reductase, Peptidy1-proly1 cis-trans isomerase A, Creatine kinase B-type,α-internexin, Spectrinαchain, Frunctocse-bisphosphate aldolase C, Guanine nucleotide-binding protein G submitα, Dynamin-1. Conclusion: we have identified a number of protein, which may be involved in stress, inflammation, apoptosis, brain repair after cerebral hemorrhage. This will help us to comprehense the pathological mechanisms of ICH at the protein level.