Identification Of Proteins Differentially Expressed In Pre-eclamptic Trophoblasts By Proteomic Analysis

Pre-eclampsia (PE) is the specific pregnant disease that complicates 5%～8% of all pregnancies, resulting in significant perinatal morbidity and mortality. The disease is manifest in the third trimester of pregnancy with a wide variety of maternal symptoms, of which hypertension, proteinuria, and edema are late manifestations of a multifactorial, multi-systemic disorder initiated in early pregnancy.But the etiopathogenisis and pathogenesy of PE is unknown, so PE remains difficulty in early diagnosis to optimize the opportunity for prevent and treatment. More and more evidences show that the pathogenesy of PE is relevant to disfunction of trophoblast, for example impaired invasion, aberrant adherence factor and cytokine, disordered immunity system, over-apoptosis, viral infection, et al.Objective: To identify differential trophoblastic proteins in normotensive pregnant women and pre-eclamptic patients by proteomic analysis in order to further elucidate the complex molecular mechanisms of PE. Methods: To isolate, purify and identify trophoblasts from human normotensive pregnant and pre-eclamptic patient's placentas. Then we prepared trophoblastic proteins. The expression of proteins was determined by sliver staining after two-dimensional polyacrylamide gel...