Research The Appetite And Related Neuropeptides Effects Of Eliminating Wetness-evil And Stomach-harmonizing Method In Treating Liver-against-stomach Rats

Objective: Observe the effects of appetite, orexin A and leptin on the eliminating wetness-evil and Stomach-harmonizing method in treating liver-against-stomach rats, to explore its relationship. Preliminary attempt from another point of view to explore the treatment of liver-against-stomach in the wetness-evil change impacts on the possible mechanisms of appetite.Methods: A total of 58 male Wistar rats, with body weight ranging from 200 to 240 g, provide by the Experimental Animal Center of Hebei Medical University. First, rats were randomly divided into model checking groupⅠ(n = 8) and experimental groupⅡ(n=50). randomly divided model checking groupⅠinto the model checking 1 group(n=4) and model checking 2 group(n=4).Ⅱgroup were treated with the randomized block design is divided into 5 groups. 10 rats in each group, the normal group (A), angered model control group (B), drug treatment of low-dose group (C), drug treatment dose group (D), drug treatment of high-dose group (E). Every day at 9 am in each group give a certain amount of rat food provide by the Experimental Animal Center of Hebei Medical University, the next day at 9 am measurement of the remaining mice in each group. To model checking 2 group, angered model control group, drug treatment of low-dose group, drug treatment dose group, drug treatment of high-dose group, were modeling by rat tail pain stimulation methods. Modeling seven consecutive days, model checking 1 group and model checking 2 group after 12 hours fasting for model testing. During the test model the other groups to continue to stimulate. Model testing method: First, from behavioral observation, stimulated rats drinking, eating and weight significantly reduced, even don't want to eat. Look at the performance of a sleepy state, and appear darker hair, brown-like changes; Second, up from the laboratory testing, to eight test rats were decapitated, After the rats were decapitated rapidly save the blood samples, using Elisa kit for determination of serum leptin levels. Test results comparing, model checking 2 group of serum leptin levels higher than model checking 1 group. Test results of the two-sample t test between the two groups the difference was statistically significant, modeling success. Stop the anger stimulus. The normal group and angered model control group rats were 2ml distilled water intragastric administration per day for sham treatment. The drug treatment group began the daily application of different doses of self-made Huzhuohewei decoction intragastric administration. The decoction condensed into liquid 1 /10, cooking by the Hebei Traditional Chinese Medicine Institute. C group by the adult dose of 10 times intragastric administration to rats, 2 ml each (0.5ml concentrated liquid medicine plus 1.5ml distilled water); D group by the adult dose of 20 times intragastric administration to rats, 2 ml each (1ml concentrated liquid medicine plus 1ml distilled water); E group by the adult dose of 40 times intragastric administration to rats, 2 ml concentrated liquid medicine each, gavage once a day in each group. After seven consecutive days, on the same day the morning was decapitated for all rats. After decapitation quickly isolated hypothalamus and save blood samples on the ice box, Using Elisa kit for determination of serum leptin levels. Using RIA kit for determination of Plasma and hypothalamus tissues of orexin A levels. The purpose of this study is that, according to different doses of the drug treatment group and control group test results, observation the effect of drug treatment on rats with appetite, orexin A and leptin levels. Preliminary attempt from theory to explore the treatment of liver-against-stomach in the gloomy and wetness-evil change impacts on the possible mechanisms of appetite.Results: 1. Appetite changes: Normal group, has always been relatively stable food intake; modeling phase angered model control group and the drug treatment groups, appetite significantly reduced, a downward trend evident; Treatment period angered model control rats continued to maintain a low appetite and food intake status, food intake to restore not obvious. drug treatment of low-dose group rats food intake gradually picked up, but the average daily food intake is still far lower than the normal group. Drug treatment of dose group rats food intake gradually a steady upswing. To the end of the treatment period have been restored to near normal levels of the rats daily food intake. Drug treatment of high-dose group recovery trend is clearly, after treatment, food intake has even later than normal daily food intake in rats.2. Weight changes: After the initial group: body weight between the rats in each group no significant difference (P> 0.05). Modeling success: weight of rats in each group there was significant difference (P <0.01). Pairwise comparison results indicate that normal body weight with the other groups were significantly different (P <0.001). In addition to the normal group body weight among the other groups there was no significant difference (P> 0.05). From the mean look to the body weight in each stimulate group significantly lower than normal group (224.10±10.34g). After treatment: body weight in each group there was significant difference in contrast (P <0.01). Pairwise comparison results indicate that the normal group (A) with drug treatment of high-dose group (E) and the drug treatment dose group (D) with drug treatment of high-dose group body weight was no significant difference (P> 0.05 ). A group and D group compared 0.01