The Research For Relation Between The Th1/Th2 In Blood Plasma And The Change Of Microecosystem In Children With Acute Attack Wheezing

Objective: wheezing is the universal symptom in the first year of children. Clinical observation confirms that the children wheezing and the children asthma have the same clinical situation and turnover. All of those research demonstrate that there may be has sequential and common pathogenesy in both diseases. Accordingly, if we can decrease the morbility of infancy wheezing, we may ba can decrease the morbility of children asthma. In this study, by measuring serum levels of INF-γand IL-4 in children with acute attack wheezing, we will study the role of INF-γand IL-4 in the children wheezing pathogenesis. By measuring the change of intestinal bacteria and investigating the relation between the B/E and IL-4/INF-γ, then we try hard to illuminate effective of intestinal normal bacteria and Th cells in children with acute attack wheezing. Then we can provide a determinate theoretical basis for preservation and treatment of children wheezing through adjunction probiotics.Methods: Forty-seven children with low respiratory infections following wheezing (Records is A group) and forty children with low respiratory infections (Records is B group) from the pediatric department in the Third Hospital Of Hebei Medical University and thirty healthy children(14male and 16 female, age 2.2±0.25 years) were selected in the study during August in 2009 to February in 2010. A group divide to A1group and A2 group. A1 group has 22 examples who didn,t use antibiotic before hospitalization(8 male and 14 female, age 2.1±0.26 years). A2 group has 25 examples who used antibiotic before hospitalization(14 male and 11 female, age 1.9±0.19 years). B group divide to B1group and B2 group. B1 group has 18 examples who didn,t use antibiotic before hospitalization(8 male and 10 female, age 2.2±0.24 years). B2 group has 22 examples who used antibiotic before hospitalization(9 male and 13 female, age 1.8±0.21 years). The sexes, the age, the body weight, the height, the birth way, the feeding way and the life region between the three groups do not have the significance difference. A group and B group in hospital same day and the healthy-control group at early morning were resting blood sampling 2ml, simultaneously assemble Fresh fecal sample. All specimens were separated of serum by centrifugal immediately. The serum asmples were stored at -80℃refrigerator until detected. Use the way of ELISA measure the level of IL-4,INF-γand TGF-β1,and account the ratios of INF-γ/IL-4. 16S rRNA copy numbers of bacterial genome DNA extracted from fecal samples were quantified by real-time PCR to analyze bacterial amount.Results:1 Change of intestinal flora in each groupThe quantity of bifidobacteria and B/E in A1 group is lower than that in C group, the difference has statistically significant (P<0.05). The quantity of bifidobacteria and B/E in B1 group is lower than that in C group, the difference has statistically significant (P<0.05). The quantity of bifidobacteria and B/E in B2 group is lower than that in B1 group, the difference has statistically significant (P<0.05). There was no difference between A1 and A2 group(P>0.05).2 The concentrations of IL-4 and INF-γin hematoplasma in each group2.1 the level of INF-γin hematoplasma: The level of INF-γin A1 group is lower than that in B1 and C group,the difference was statistically significant (P<0.05). The level of INF-γin B 1 group is lower than that in C group,the difference was statistically significant (P<0.05).2.2 the level of IL-4 in hematoplasma: The level of IL-4 in A1 group is higher than that in B1 and C group,the difference was statistically significant (P<0.05). The level of IL-4 in B 1 group is higher than that in C group,the difference was statistically significant (P<0.05).2.3 the level of INF-γ/IL-4 in hematoplasma: The level of INF-γ/IL-4 in A1 group is lower than that in B1 and C group,the difference was statistically significant (P<0.05). The level of INF-γ/IL-4 in B 1 group is lower than that in C group,the difference was statistically significant (P<0.05).3 There is a negative correlation between IL-4 and TGF-β1 in A1 and A2 group (r1=-0.51, r2=-0.69; P<0.05). INF-γand TGF-β1 do not showed correlation.4 INF-γ/IL-4 and B/E showed a positive correlation in A1 group(r=0.43,P<0.05).5 The numbers of WBC and the level of CRP in A1group were lower than that in B1 group,the difference has statistically significant (P<0.05).Conclusion:1 Serum INF-γlevel in wheezing children with acute episode was decreased,but Serum IL-4 level was increased. wheezing children was in Th1/Th2 disbalance. This participate the mechanism of wheezing.2 In the period of wheezing with acute episode, intestinal microbio has confused condition. The appearance was decrease of bifidobacteria and B/E. This explain wheezing with acute episode can effect the ecotope of intestinal microecology.3 The balance of Intestinal flora can interact the balance of Th1/Th2. In the period of wheezing with acute episode, both sides| influence each other, the appearance is the decrease of B/E and the disbalance of INF-γ/IL-4.So the relationship between both sides is influence each other.4 Our investigation can provid an significant theoretical basis for probiotics preservation and treatment children wheezing through adjunction probiotics.