Study About Variations Of Intestinal Flora And The Ralation With Plasma Interleukin-17 In Wheezing Attack Children

Objective:Wheezing is the common symptom of respiratory diseas-e in infancy. In recent years, epidemiological survey show that the incid-ence of infantile wheezing has been increasing year by year,and it influ-ences children's life and quality of life seriously.So far,most studies hav-e investigated association of gut microbiota with allergic diseases such as eczema and allergic sensitization,but studies investigating the ralation between intestinal flora and wheezing in childhood are still limited.IL-17 and Th17 cells are responsible for the development of autoimmune dis-eases such as murine arthritis and encephalomyelitis,which have classical-ly been considered to be Th1-mediated disorders.They may also contri-bute to the pathogenesis of classically recognized Th2-mediated allergic disorders.We study the changes of fecal bifidobacteria and bifidobacteri-a/E.coli(B/E),the changes of plasma IL-17 level in wheezy children and the ralation of fecal B/E and plasma IL-17,aimming at investigating the role of intestinal flora and IL-17 in pathogenesis of wheezing.This stu-dy could provide theoretical basis for reasonable application probiotics a-nd new ideas for IL-17 becoming a new target to prevent and treat w-heezing and asthma in childhood on clinical.Method: 47 children with lower respiratory infections accompanied by wheezing are selected,including 22 children without the use of antibiotics and 25 children using antibiotics within five days before hospitalization.40 chilidren with lower respiratory infections not accompanied by wheezing are selected,including 18 children without the use of antibiotics and 22 children using antibiotics whthin five days before hospitalization. 30 healthy children are selected in the corresponding time period. Every group is comparable with the other group in the gender composition, the age, the weight, the feeding patterns and the living region.This experiment is divided two parts to carry on: 1 the bifidobacteria and E. coli DNA in fecal samples were detected by flurogenic quantitative polymerase chain reaction(FQ-PCR);2 IL-17 in plasma were detected by enzyme linked immunosorbent assay. Date was analyzed by SPSS 13.0 statistical software. Statistical analysis was performed using two independent sample means of comparison and linear regression correlation.P<0.05 were considered as significant.Result:1 Comparision of quantity of fecal microorganisms in five groupsThe concentration of fecal bifidobacteria, B/E values in A1 and B1 group were lower than those in C group, the difference was statistically significant (P <0.05). The difference of concentration of bifidobacteria and B/E value between A1 and B1 group, between A1 and A2 group was not statistically significant (P> 0.05); The bifidobacteria concentration and B/E value in B2 group were lower than those in B1 group, the difference was statistically significant (P <0.05) (see Table 2).2 The different level of IL-17 in plasmaThe IL-17 level in plasma in A1 and B1 group was respectively higher than those in C group, the difference was statistically significant (P <0.05).The IL-17 level in plasma in A1 group was lower than those in B1 group, the difference was statistically significant (P <0.05).The IL-17 level in plasma in A1 and A2 group were not different (P> 0.05). The IL-17 level in plasma in B1 and B2 group were not different (P> 0.05)(see Table 3).3 The correlation analysis between B/E in fecal samples and IL-17 in plasma in children with wheezing attack showed, B/E in fecal samples was not linear correlated with IL-17 in plasma in A1 group(see Fig.5 ).4 The correlation analysis between TGF-β1 and IL-17 in plasma in children with wheezing attack showed, TGF-β1 was positively correlated with the IL-17 in plasma in A1 group (r=0.966,P<0.05).The regression equation was: Y=2.499 X-16.134(see Fig.6 ).Conclusion: 1 The intestinal flora of children with wheezing attack diordered, manifested as reduction in the quantity of bifidobacteria and B/E.This implied intestinal microbial colonization resistance decreased,and we made comprehensive analysis.2 The intestinal flora in children with lower respiratory infections, regardless of whether accompanied by wheezing,disturbanced. B/E value in fecal samples in children without wheezing and wheezing were not different.3 IL-17 in plasma in children with wheezing attack was involved in the disease process, and was positively correlated with TGF-β1.This indicated both Treg cells and Th17 cells played a role in the pathogenesis of acute wheezing attack.4 From this study,we didn't find the direct ralation between intestinal microflora patterns and IL-17 level in plasma in children with acute wheezing attack.