Effects Of Allicin On Cholesterol Exllusion And Expression Of ABCG5 And ABCG8 In Intestinal Epithelial Cell

OBJECTIVE To explore the effects of allicin on expression of intestinal cholesterol transport related genes of ApoE-/- Mice and caco-2 cell,so that we can illuminate the role of Allicin on atherogenesis.METHODS 48 male apoE-/- mice,6 weeks old, randomly divided into four groups: normal food group[normal food+PBS 0.1ml/2d, intraperitoneal injection (IP)], high fat high cholesterol group (high fat high cholesterol food+PBS 0.1ml/2d, IP); high fat high cholesterol and Allicin group (high fat high cholesterol+allicin 0.1ml/2d, IP). normal food and allicin group (normal food+allicin 0.1ml/2d, IP).Animals from high fat high cholesterol group and high fat high cholesterol and allicin group were fed with western food which concluding 10% fat and 2% cholesterol. After feeding 12 weeks, all mice were anesthetized by 0.2～0.3ml Urethane (20%) and removed eyeball in order to obtain blood preparation. Serum triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) were determined by commercially enzymatic methods. the whole aorta were dyed with SudanⅣto examine the atherosclerotic lesion area.The Hematoxylin/Eosin dyeing of paraffin section was used to detect the area of atherosclerotic plaque of apoE-/- mice aortic root transaction. The expression of ABCG5 and ABCG8 measured by immunofluorescence staining and RT-PCR. Caco-2 cell was divided into four groups:control group, caco-2 cell model group (treat cell with cholesterol),caco-2 cell model and allicin group (treat cell with cholesterol and allicin). caco-2 and allicin group(treat cell with allicin). TC measured by HPLC. mRNA and protein expression was analyzed by semi-quantitative RT-PCR and Western blot, respectively. RESULTS Compared with model group, the level of TC, TG, LDL-C was lower in allicin treated group (p<0.05). HDL-C was up-regulated but not markedly changed. The aorta atherosclerotic lesion area of allicin treat group is smaller than model group. HPLC illustrate TC was lower in allicin treated group. Oil red O staining illustrate Model group were higher in the lipidoses of caco-2 cell than allicin treated group. The expression of ABCG5/ABCG8 mRNA and protein of allicin treat group were higher to other groups in animal and cell (p<0.05 or p<0.01).CONCLUSIONS Atherosclerotic lesions in apoE-/- mice were attenuated by long term administration of allicin. It can decrease serum lipid level, atherotic area of aorta and aortic sinus. The possible mechanisms of this action for allicin are associated with the up-regulation the expression of ABCG5/8 in ApoE-/- Mice intestinal and caco-2 cell.