Ibrolipim Increases The Expression Of ABCA1/G1 By LXRα Signaling Pathway In THP-1 Macrophage-Derived Foam Cells

OBJECTIVE: The objective of this study was to determine the effects and potential mechanisms of Ibrolipim on cholesterol efflux from human macrophage foam cells, which may play a critical role in atherogenesis.MEHTODS: In the present study, Human THP-1 cells pre-incubated oxLDL served as foam cell models. Real-time quantitative PCR and western blot were conducted to determine the effects of Ibrolipim on the expression of ABCA1 and ABCG1 and Liver X receptorα(LXRα). Liquid scintillation counting and high performance liquid chromatography assays were used to test cellular cholesterol efflux and cholesterol content.RESULTS: Ibrolipim significantly increased cholesterol efflux from THP-1 macrophage-derived foam cell to apoA-I or HDL. Moreover,it markedly increased the expression of ATP-binding membrane cassette transporter A-1 (ABCA1) and ABCG1, In addition, LXRαwas also up-regulated by Ibrolipim treatment. And LXRαsmall interfering RNA completely abolished the promotion effect which was induced by Ibrolipim.CONCLUSION: Ibrolipim increased ABCA1 and ABCG1 expression and inhibited cholesterol efflux, which was mediated by LXRαsignaling pathway.