The Study Of Pfos's Hepatorenal Toxicity And Lycopene's Protection Function In Rats

Objective:To study the hepatorenal toxicity of the perfluorooctane sulfonate(PFOS) in rat and the lycopene's protection function of it. To provide scientific evidence for further clarifying PFOS's hepatorenal toxicity and mechanism and expanding the lycopene's application.Method:48 healthy SD rats were averagely divided into 8 groups randomly including control group,low dose PFOS group, middle dose PFOS group, high dose PFOS group, LP protected group, LP protected low dose PFOS group, LP protected middle dose PFOS group and LP protected high dose PFOS group. The four PFOS groups were respectively fed fodder added different dose PFOS(0, 5, 25 and 125mg/kg fodder), keeping on fed 2 months and they were also lavaged solvent(1%CMC-Na) for keeping on 2 months, five days/week and 1/day. While four protected groups were fed PFOS as same as the four PFOS groups, but they were lavaged lycopene 20mg/kg body weight. 24 hours after last gastric perfusion, Blood was taken from venous plexus of rats'eye sockets and then killed them. The level of blood serum ALT, ALP, AST, UREA, CRE, UA were detected by automatic biochemical analyzer. The rats'liver and kidney were fetched and computed their organ coefficients. Each rat's half liver and kidney were used to doing conventional pathology section, HE staining to observe their morphous changes. The other half were homogenised and centrifuged, and the supernatant was used to detect the level of MDA, SOD, GSH, GSH-Px by Nitrite-kit method or DTNB method.Results:1. The growth of rats in middle and high dose PFOS group was slower than that of in low dose group, and as increasing of PFOS dose, the inhibitive effect of weight gain become more clear(P<0.05). The rats weight growth in LP protected group were improved compared with same dose PFOS rats without LP protected, but didn't have statistical significance (P>0.05).2. Middle and high PFOS group rats's liver and kidney organ coefficient were lower than control group, and the difference had statistical significance(P<0.05), and they had dose-effect relation. The liver and kidney organ coefficient of LP protected group rats were higher than the same dose PFOS group, there was statistical significance(P<0.05).3. Liver tissue section showed that compared with control group or LP protected group, Liver tissue in low dose PFOS group was normal, but in middle and high dose PFOS group it had obvious cell edema and diffuse steatosis, and LP protected PFOS group just had slight ballooning degeneration and cytoplasmic relaxation.Kidney tissue section showed that compared with control group and LP protected group, kidney tissue in low dose PFOS group also were normal, but middle and high dose PFOS group had obvious cylindruria and protein exudation, and LP protected PFOS group just had few inflammatory cell infiltrate.4. Compared with control group, middle and high dose PFOS group rats'ALT, ALP, AST, CREA, UREA, and UA increased, the difference had statistical significance(P<0.05). And the LP protected group rats'ALT, ALP, AST, CREA, UREA, and UA were lower than PFOS groups, the difference had statistical significance (P<0.05).5. Compared with control group, the rats'liver and kidney tissue MDA, GSH in middle and high dose PFOS group increased, GSH-Px, SOD activity dropped, there were statistical significance(P<0.05). And the rats'liver and kidney tissue MDA, GSH in the LP protected group were lower than that in PFOS group, but GSH-Px, SOD activity were higher, the difference had statistical significance(P<0.05).Conclusions:1. PFOS may damage rats'liver and kidney.2. 20mg/Kg.bw LP can antagonize PFOS's hepatorenal toxicity.3.The LP's protection mechanism for PFOS's hepatorenal toxicity may be antioxidation.