PFOS Effect On The Immunotoxicity Of Rats And The Protection Of Lycopene

Objective:To evaluate potential toxicity effects of Perfluorooctane Sulfonate (PFOS) on the immune injury and intervention effects of Lycopene (LP), clarify the damage of PFOS on rats and its mechanism, and provide experimental evidence of protective effects of Lycopene (LP), a novel work had been carried out by exposing rats to the different levels of PFOS and LP.Method:The 48 healthy SD rats (male and female in half) were divided into 8 groups: control group; low-dose, middle-dose and high-dose PFOS group; LP protection group; LP protection group with low-dose PFOS; LP protection group with middle-dose PFOS; LP protection group with high-dose PFOS. Both Control group and the LP protection groups were given fodder with 2% Tween-80, the experimental groups were given the treatment fodder with dose 5, 25, 125 mg/kg PFOS respectively, control group and low, middle and high doses PFOS groups were exposured with 1% CMC-Na solution, LP and LP protection groups with the low, middle and high dose PFOS were given the 20mg/kg·bw LP suspension for 2 months continuously (once a day, 5d as a week). all the rats were killed in 24 hours after the last gastric perfusion, the blood from the orbital venous plexus was collected then serum was prepared to determinate the IFN-γ, IL-4 content using enzyme-linked immunosorbent assay (ELISA), analysis lymphocytes subgroup by FCM, drop pieces method to determine phagocyte function,calculate organic coefficient extracting thymus and cipher; General Pathology section was made to observe the spleen morphology with HE staining;spleen cell suspension was prepared, Hemolytic plaque method was applied to evaluate the antibody forming cells with, MTT method to determine lymphocytes proliferation, LDH release method to determine NK cell activity。Results:1. The body weight in middle and high dose PFOS group lower than those in control group, PFOS could reduce body weight, the degree of inhibition was related with the administrated dose of PFOS (P<0.05); After adding LP, the weight of rats in LP protection group and LP protection groups with low, middle and high dose PFOS higher than the same dose PFOS group, but no statistical significance (P>0.05)2. The spleen and thymus coefficient in middle and high dose PFOS group lower than those in the control group (P<0.05), and there was a dose-response relationship; Compared with the same dose PFOS group by adding LP, LP protection group with middle and high doses PFOS, the spleen coefficient increased, the difference was statistically significant (P<0.05), The thymus coefficient slightly also increased, but no statistical significance (P>0.05).3.The antibody-forming cells in the low, middle and high dose PFOS groups lower than those in the control group (P<0.05), and there was a dose-response relationship; Compared with the same dose PFOS group by adding LP, LP protection group with low,middle doses PFOS, antibody-forming cells increased and there was significantly different (P<0.05).4. The lymphocyte proliferation, CD4 + T cells and CD4 + / CD8 + in the middle and high dose PFOS groups lower than those in the control group (P<0.05) , and there was a dose-response relationship; Compared with the same dose PFOS group by adding LP, LP protection group with low, middle and high doses PFOS, the lymphocyte proliferation, CD4 + T cells and CD4 + / CD8 + increased(P<0.05); The mononuclear phagocytic percentage, phagocytic index and CD8 + T cells of the high dose PFOS group decreased (P<0.05) and there was significantly different (P<0.05).5. Compared with the control group, the NK cell activity in the middle and high dose PFOS group decreased (P<0.05), and there was a dose-response relationship; Compared with the same dose PFOS group by adding LP, the NK cell activity of LP protection group with low, middle doses PFOS increased and there was significantly different (P<0.05).6. The IFN-γof the middle and high dose PFOS groups were lower than that of the control group (P<0.05) and there was a dose-response relationship, The IL-4 slightly also decreased, but no statistical significance(P>0.05); Compared with the same dose PFOS group by adding LP, the IFN-γand IL-4 of LP protection group with low, middle and high doses PFOS increased and there was significantly different (P<0.05).Conclusion:1. PFOS could induce the immune toxicity injury of rats.2. lycopene(20mg/kg·bw) might protect the rats from immune toxicity injury caused by PFOS.