The Protective Effects Of Hypertonic Sodium Chloride Hydroxyethyl Starch 40 On Rat Cerebral Ischemia/Reperfusion Injury

Western Blotting检测显示:与A组相比,B组NSE,HSP70蛋白表达升高(p<0.01);与B组相比,C组HSP70蛋白表达升高(p<0.05),C,D组NSE表达降低,差异有统计学意义(p<0.01)。结论1. HH40可明显缩小大鼠缺血再灌注后脑梗死体积,改善神经功能行为学评分,降低脑组织含水率,提示HH40对脑缺血再灌注损伤有明显的保护作用。2. HH40可显著降低大鼠缺血再灌注后MDA的生成,降低脑组织NO含量,iNOS活性,提高SOD,GSH-Px,Na⁺-K⁺-ATPaseå’ŒCa²⁺-Mg²⁺-ATPase的活性。表明HH40对脑缺血再灌注损伤的保护作用可能与HH40抗氧化作用,参与NO的调控,抑制iNOS表达及改善脑能量代谢障碍有关。3. Western Blotting检测显示HH40能上调大鼠缺血再灌注后脑组织HSP70蛋白的表达,降低NSE的表达,提示HH40通过提高HSP70蛋白的表达,降低NSE的表达发挥其保护作用。 ResultsCompared with group B, the volume of infarction and neurological behavioral score were both reduced in group C and D(p<0.01). In addition, water content of left brain and the level of MDA in serum and NO contents of ischemic brain tissue were also reduced in group C and D(p<0.01),while SOD activity was increased and water content of right brain was reduced in group C(p<0.05). GSH-Px, Na⁺-K⁺-ATPase and Ca2 +-Mg2 +-ATPase activities were all increased in ischemic brain tissue(p<0.01),while iNOS activity was decreased in group C(p<0.01)and D(p<0.05). Western blotting analysis showed that the expression of HSP70 protein and NSE were increased in group B compared with A(p<0.01); and the expression of NSE protein were declined in group C and D compared with group B(p<0.01); while the expression of HSP70 protein was increased markedly(p<0.01).Conclusions1. HH40 could significantly reduce the volume of cerebral infarction, improve neurological behavioral score and decrease water content of injured cerebral tissue, which shows that HH40 has a protective effect on cerebral I/R in vivo.2. After I/R, the MDA formation in rat was significantly reduced, while SOD, GSH-Px, Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase activities were significantly increased by HH40 infusion, which indicates that the protective effect of HH40 on cerebral I/R may be related to its antioxidant effects, regulating NO levels, inhibiting iNOS activity and improving cerebral energy metabolism.3. Western blotting analysis showed that HH40 could increase the expression of HSP70 protein and decrease the NSE expression, which suggests that the mechanism of HH40 may be related to the increase of HSP70 expression and the decrease of NSE .