Comparison Between Hypertonic Saline, Sodium Chloride Hydroxyethyl Starch And Mannitol Of Curative Effect In Super Early Cerebral Infarction In SD Rats(Summary)

Objectiv Study of the treatment effection apply20%Mannitol,7.5%HS, HS-HES and HES on acute cerebral infarction secondary cerebral edema in SD rats and to provide the experimental basis for the clinical therapy.Methods Male Sprague-Dawley rats weighing2-3month and250-300g were obtained from the Experimental Animal Center of the Kunming Medical University.Then randomly divided into six group sham operation group (n=8), infarction group (n=8),7.5%hypertonic saline group (n=8), hyperosmotic hydroxyethyl starch200/0.5and sodium chloride injection group (n=8), hydroxyethyl starch130/0.4and sodium chloride injection group (n=8), and20%mannitol group (n=8),.Iniect into the tail vein and maintain the injection time is larger than15min. Finally through the Three methods for comparison of four kinds of drug treatment, respectively Zea-Longa score, the brain tissue HE staining method,and Western blot.Results At first hyperosmotic hydroxyethyl starch200/0.5and sodium chloride injection improve to the neurological function infarction and clear edema of acute cerebral has obvious than mannitol in SD rats.7.5%hypertonic saline, hyperosmotic hydroxyethyl starch200/0.5and sodium chloride injection, hydroxyethyl starch130/0.4and sodium chloride injection and20%mannitol are all contributions to down-regulation of AQP4expression, but there is no significant difference between them.Conclusions Each time point of Zea longa score by K-W test with significant differences among the groups and group witch AQP4gray level by the ANOVA, in addition to infarction group showed no significant difference between the treatment group and brain edema were reduced or even disappear.