Evodiamine Induces Caspase-dependent Apoptosis And S Phase Arrest In Human Colon Lovo Cells

Objective: This study is performed to elucidate that evodiamine inhibited proliferation of human colon lovo cells in vitro and in vivo, and to research on mechanism responsible for the inhibition.Methods: The effect of evodiamine on proliferation of cells was obtained by MTT assay and lovo human colon tumor xenografts. Cell cycle arrest and cell apoptosis were measured by flow cytometry and tumor apoptosis was measured by western blot. The translocation of AIF and endoG was analyzed by immunocytochemistry. The expressions of procaspase-8,-9,-3, Bcl-2, Bax, CyclinA, CDK2, CyclinB1, CDK1, cdc25c were examined by western blot. The activities of caspase-8,-9,-3 were examined by caspase-8,-9,-3 activity assay kits.Results: Evodiamine inhibited proliferation lovo cells in vitro and in vivo. Evodiamine-induced apoptosis was due to dose- and time- dependent decreased expressions of procaspase-8,-9,-3 and increased activity of caspase-8,-9,-3, accompanied with a decline in the Bcl-2/Bax ratio, but not the translocation of AIF and endoG. Evodiamine-induced S arrest was associated with a marked decrease in the protein expressions of cyclinA,CDK2,cdc25c in lovo cells.Conclusions: These findings indicate that evodiamine inhibits proliferation of human colon lovo cells in vitro and in vivo through caspase-dependent apoptosis and S arrest.