Studies Of Evodiamine-induced Cell Cycle Arrest On The Human Cervical Cancer HeLa Cells

Cancer is a major killer of human health. The development of tumor is closely related to the disorders of normal cell cycle control mechanisms. Studies found the expression of cell cycle regulators,related to cell cycle changes, is disordered in many tumor cells. Therefore, take cell cycle regulators for targets and develop drugs , those provide a new program to cancer treatment.Evodiamine is an alkaloid extracted from traditional Chinese medicine Wuzhuyu . From the laboratory basic research, evodiamine can induce cervical carcinoma HeLa cells to apoptosis and cycle arrest ,but the mechanism is still unclear. In this paper, we research the initial molecular mechanism of cell cycle arrest induced by evodiamine. We hope that we can have a deep understand of the anti-tumor effect of evodiamine, and provide some reference to its research , its development and cancer treatment .First, evodiamine induced HeLa cells with the concentration of 0.1,1,10μg / ml ,then measured by crystal violet staining to verify its cytotoxicity. From the result ,we can know evodiamine has high cytotoxicity, and their cytotoxicity has time and concentration dependent obviously. Next, the concentration of 1μg/ml of evodiamine is selected to induced HeLa cells with 0,4,8,12,16,20,24 hours of proliferation. cell cycle of HeLa cells detected by flow cytometry was arrested at G2 / M phase. In order to understand the molecular mechanism of G2 / M arrest caused by evodiamine. the expression of cyclinB1mRNA was researched , and we found that the expression of cyclinB1mRNA was inhibited. p21mRNA expression was raised thought the further researchIn summary, we have come to the preliminary conclusion: Evodiamine is up the expression of p21mRNA,then may lead to the increase of p21 protein, which mediated the inhibition of the expression of cyclinB1mRNA.And cyclinB1 protein expression may be inhibited, thus make Hela cells arrested in G2 / M phase.