Preparation And Prelinminary Cytology Investigation Of IL-2 Targeted 5-FURP Liposome

Objective:Selecting 5-FURP as model drug,preparaing IL-2-5-FURP-L, studying the technology of preparation and investigating the inhibition of the growth of cells with corresponding receptors by IL-2-5-FURP-L.Methods:The reverse phase evaporation was used to prepare IL-2-5-FURP-L.The stability of the liposome was determined by the entrapment and IL-2 contents after they were stored at 4℃and 25℃respectively for one month.MTT method was used to investigate the liposome's targeting and anti-cancer on human T cell lymphomas Hut-102 cells.Results:Through orthogonal experimental design, one optimum recipes of IL-2-5-FURP-L was founded:Drug/EPC 1:20,EPC/Ch 3:1,sonication time 8 min,preparation temperature 45℃.Under the optimal formulation,the entrapment efficiency of IL-2-5-FURP-L was 91.30%.The liposome observed through transmission electron microscopy presented distribution of size uniformity and the average diameter was about 180nm.MTT tests showed that IL-2-5-FURP-L was able to suppress the growth of tumor cells depending on dosages.The inhibition rate of free 5-FURP, 5-FURP-L and IL-2-5-FURP-L on human T cell lymphomas Hut-102 cells were 59.19%,64.45% and 97.34%.Conclusions:UV method was adopted to detect IL-2 contents,entrap- ment efficiency of IL-2-5-FURP-L,which was accurate,fast and convenient. Stability experiment showed,liposome was comparatively stable at the storage temperature of 4℃.The drug releasing experiment in vitro showed the liposome had released more slowly than free drug.Cytology investigation showed that IL-2-5-FURP-L could inhibit the growth of Hut-102 cells better than free 5-FURP and 5-FURP-L,IL-2 as carrier on TDDS to carry out targeting release.So IL-2-5-FURP-L had better antitumor activity.