| Objective To evaluate the feasibility and safety of co-transplantation of SVF-PLGA with fat complex on the construction of tissue engineered implant.Methods 40 adult New Zealand male rabbits were randomly divided into four groups according to different implants. In the experimental group A (n=10), implant was constructed by the used of SVF-PLGA with fat complex and in group B SVF-PLGA complex buried by adipose tissue was used. In the control group C(n=10) only PLGA and in the group D(n=10), SVF-PLGA complex was used. All the complexes were transplanted between skin and fascia superficial. Tissue samples obtained were analyzed with gross observation, HE stain and red oil stain at 2 and 4 months postoperatively.Results The tissue engineered implant was mainly vascularized adipose tissue without tumor cell. PLGA scaffolds were degraded completely 4 months later. the survival rate of adipose in experimental group B was higher than in the group A and in the control group B.Conclusion SVF-Lipo-PLGA complex on soft tissue augmentation is safe, maneuverable, time-saving, and with the great advantage of high survival rate of adipose and omitting the step of amplification in vitro.
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