The Effect Of Tumor Chemosensitivity Of LMP1 Knockdown By RNA Interference

Malignant tumor continues to be the leading cause of human mortality. One of the obstacles of chemotherapy is drug resistance. How to use chemotherapy combined with gene therapy to enhance chemosensitivity has been paid much attention recently.On the basis of siRNA targeting latent membrane protein 1(LMP1) specifically and successfully, chemosensitivity of lymphoma B95.8 cells is studied in this paper.1. According to LMP1(NC-007605) sequence in GenBank, we selected two specific small interference RNA(siRNA) fragments targeting LMP1. Knockdown effect was tested by RT-PCR and Western-Blot respectively. Compared to the control groups, both mRNA and protein of LMP1 dramatically lower, indicating that these two siRNA fragmens were successfully inhibit the expression of LMP1 in B95.8 cells.2. Cell cycle of different treatment was then analyzed by flow cytometry. The results showed that LMP1 knockdown caused G0/G1 phase arrest.3. The result of drug effect experiment showed the viability of cells was decreased with the concentration of cisplatin increased, and also with the time extened, indicating that cell viability to cisplatin was on concentration-and time-dependent manner.4. MTT assay showed that IC50 value was decreased in LMP1 knockdown group when compared with control, which indicated that inhibition of LMP1 expression increased the chemosensitivity of B95.8 cells to cisplatin.