Effects Of MSCs Transplantation On Vascular Restenosis Of Balloon Injury And Cardiac Function After Myocardial Infarction

Objective Although the effectiveness of MSCs transplantation on myocardial infarction has been confirmed, the effects on vascular restenosis after PCI remains controversial while the improvement of the cardiac function after myocardial infarction. The study proposed by establishing a similar clinically PCI treatment after myocardial infarction of animals model, namely, the double model of myocardial infarction reperfusion and atherosclerotic stenosis carotid artery by balloon injury. A parallel move to the observation the effects of MSCs transplantation on the injured vascular restenosis after myocardial infarction of rabbit and cardiac function, investigate the possible mechanism of MSCs transplantation treatment in myocardial infarction and whether or not to effect on injure vascular restenosis. Provide meaningful based on animal experiments for clinically prevention and treatment of vascular restenosis after PCI.Methods Rabbits atherosclerotic carotid artery stenosis model were established, on that basis, synchronous schedule in establish rabbit model of myocardial infarction reperfusion and carotid artery by balloon injury. Rabbit model were randomly divided into MSCs transplantation group and control group. Transplanted group was received intravenous transplantation of MSCs and control group was only received intravenous equal-volume PBS solution. One week after MSCs transplantation, the injured carotid arteries, the uninjured carotid arteries and infarction myocardium and surrounding normal the myocardium was harvested. The homing of MSCs was observed. Two weeks after MSCs transplantation, the vascular tissue was harvested and the expression of CD31, PCNA and NF-κBp65 were examined by immunohistochemical staining. Four weeks after MSCs transplantation, assessment of echocardiography and angiography of bilateral carotid arteries were performed before sacrifice. The hearts and carotid arteries were harvested and myocardial infarct size was assessed by TTC staining, Morphometric analysis of the hearts and carotid artery by hematoxylin and eosin staining.Results One week after MSCs transplantation, the DAPI-labeled MSCs could be detected in myocardial infarction and injured intima.Two weeks after MSCs transplantation, CD31 continues expression in intima of MSCs transplantation group, while the control group did not express CD31. expression of PCNA and NF-κBp65 in MSCs transplantation group is lower significantly than control group (P< 0.01).Four weeks after MSCs transplantation, MSCs transplantation group improved cardiac function, reduced myocardial infarct size, significantly increased capillary density around the myocardial infarction compared with the control group (P<0.01), and neointimal area, neointima/media area and restenosis of MSCs transplantation group were significantly lower than the control group (P<0.01).Conclusion MSCs transplantation significantly increased capillary density around the myocardial infarction, alleviated the infarcted myocardium fibroelastosis, reduced myocardial infarct size, raised ejection fraction and improves cardiac function. Meanwhile, MSCs transplantation played significant roles in promotion repair of injured vessels, suppression of neointimal hyperplasia and reduction the restenosis of injuryed vessels.